反相高效液相色谱法同时分离和测定多种头孢菌素  

The separation of cephalosporins by RP-HPLC method simultaneously

作　　者：赵军[1] 朱晨[2] 韩玲[1] 

机构地区：[1]山东大学化学与化工学院,山东济南250100 [2]山东省环境监测中心站,山东济南250013

出　　处：《山东大学学报（理学版）》2006年第4期145-147,151,共4页Journal of Shandong University(Natural Science)

摘　　要：建立了同时分离测定6种临床最常用的头孢菌素（头孢他啶、头孢拉定、头孢氨苄、头孢哌酮、头孢唑林、头孢噻肟）的高效液相色谱法．采用SHIM-PACK VP-ODS（150×4．6mml．D．）色谱柱，以乙腈及0．05mol·L^-1 HAC—NaAC缓冲溶液（pH=4．0）组成流动相梯度淋洗，254nm紫外检测，在15min内分离并测定了上述6种头孢菌素．6种药物最低检出限均可达到0．20μg·mL^-1．将该法用于尿样中药物含量的测定取得了满意结果．6种药物在尿样中回收率为92．4％-96．1％．实验证明该方法操作简便、快速灵敏、准确．An RP-HPLC method was founded, which could separate several cephalosporins under the same conditions simultaneously. Six drugs, ceftazidime, cephradine, cephalexin, cefotaxime, cefoperazone and cefazolin, were determined using ODS column, acetonitrile-HAC-NaAC buffer （pH = 4.0） as mobile phase, and UV （λmax = 254 nm）. These six drugs could be separated from each other in 15 minutes. The detect limits of these six drugs are all 0.20 μg· mL^-1. The drug amounts in pharmaceutical formulations in haman urine were determined by the proposed method. The results are satisfactory. The recovery rates of six drugs in human urine are all higher than 92.4% . It is proved that this method is simple, fast and sensitive.

关 键 词：高效液相色谱法 Β-内酰胺类抗生素 头孢菌素 

分 类 号：O557.7[理学—热学与物质分子运动论] R917[理学—物理]