补锌糖尿病大鼠肝脏糖脂代谢相关基因表达  

Expressions of genes related to carbohydrate and lipid metabolisms in diabetic rats with zinc supplementation

作　　者：孙忠[1] 吴蕴棠[1] 张万起[1] 赵娜[1] 王夏[2] 王永明[1] 

机构地区：[1]天津医科大学公共卫生学院,天津300070 [2]中国科学院微生物研究所

出　　处：《中国公共卫生》2006年第9期1108-1110,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30471459);天津市自然科学基金(033611611)

摘　　要：目的探讨补锌对糖尿病大鼠肝脏糖脂代谢相关基因蛋白磷酸酶2A(PP2A)和磷脂酶D(PLD)表达的调控。方法对补锌糖尿病大鼠差异显示的基因片段进行克隆、测序及同源性分析;设计基因序列引物,RT-PCR检测观察各组大鼠肝脏中基因表达的变化。结果结果显示,片段Zn-4、Zn-8的碱基序列分别与PLD、PP2A同源性为100%,99%。糖尿病对照组PP2A mRNA表达量(0.5072±0.0574)低于正常对照组(1.3303±0.1855),P<0.05;糖尿病补锌组PP2A mRNA的表达量(0.7005±0.1563)与糖尿病对照组比较有所恢复(P<0.05)。糖尿病对照组PLD mRNA表达量(1.0754±0.0312)和糖尿病补锌组PLD mRNA的表达量(1.0130±0.0992)均高于正常对照组(0.7995±0.1040),P<0.05;糖尿病补锌组PLD mRNA的表达量与糖尿病对照组比较无变化(P>0.05)。结论补锌对糖尿病大鼠肝脏PP2A mRNA表达有上调作用,这可能是锌改善糖尿病糖、脂代谢紊乱的分子机制之一。Objective To study the effects of zinc supplementation on related gene expression of liver in diabetic rats. Methods cDNA fragments from former research project were cloned, sequenced and BLASTn analyzed. The RT - PCRs were made using the primers designed according to the sequence of cDNA. Results The identities between the sequence of Zn - 8 and PP2A was 99%. The expression level of DM group （0. 5072 ± 0. 0574） was obviously lower than those of normal group（ 1. 3303 ± 0. 1855, P 〈 0.05）. And The expression level of DM ＋ Zn group（0. 7005 ± 0. 1563） had some improvement compared with those of DM group（P〈0.05）.Conclusion Zinc supplementation can improve the expression of PP2A mRNA on liver in diabetic rats. It may be one of the molecular mechanisms that zinc can improve the disorders of glucose and lipids metabolism in diabetic rats.

关 键 词：(PP2A) 磷脂酶(PLD) 糖尿病大鼠 RT—PCR 基因表达 锌 

分 类 号：R587.1[医药卫生—内分泌]