猪2型圆环病毒核酸疫苗的构建、鉴定和表达  

Construction ,Identification and Expression of PCV2 Nucleotide Vaccines

作　　者：秦晓冰[1] 金宁一[1] 付明山[2] 马鸣潇[1] 尹革芬[1] 金扩世[1] 李昌[1] 

机构地区：[1]解放军军事医学科学院军事兽医研究所,吉林长春130062 [2]通辽市畜牧兽医研究所,内蒙古通辽028000

出　　处：《中国兽医学报》2006年第5期465-467,共3页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2001AA213071-4)

摘　　要：以pVAX1为真核表达载体,以猪白介素18基因、猪2型圆环病毒内蒙古株的衣壳蛋白和复制蛋白基因为目的基因,构建了pVAX1-ORF2、pVAX1-IL18-ORF2、pVAX1-ORF2-ORF1和pVAX1-IL18-ORF2-ORF1等4个重组质粒作为核酸疫苗。将这4个重组核酸质粒分别酶切鉴定后,转染BHK-21和PK-15细胞进行RT-PCR、间接免疫荧光和Western blotting检测。结果表明,重组核酸质粒构建正确,并能进行表达。Using pVAX1 as eukaryotic expressing vector,swine interkine 18 genes,PCV2（Innermongolia strain） capsid and replicated protein genes as aimed genes, 4 recombinant plasmids were contructed as nucleotide vaccines. They are named as pVAX1-ORF2, pVAX1-ORF2-ORF1, pVAX1-IL18-ORF2, pVAXI-IL18-ORF2-ORF1. The four recombinant plasmids were transfected into BHK-21 cell after enzyme-identificated,and detected by RT-PCR,indirect immunofluorescence and Western blotting. The result shows that the nucleotide vaccines were constructed correctly and expressed in cells.

关 键 词：猪2型圆环病毒 核酸疫苗 RT-PCR 间接免疫荧光 

分 类 号：S858.28[农业科学—临床兽医学] S852.65[农业科学—兽医学]