马链球菌兽疫亚种类M蛋白亚单位疫苗小鼠免疫试验  

Test of M-like Protein Vaccine against Streptococcus equi subsp. zooepidemicus in Mice

作　　者：王建[1] 刘佩红[1] 沈素芳[1] 沈莉萍[1] 王永康[1] 陆承平[2] 

机构地区：[1]上海市畜牧兽医站,上海201103 [2]南京农业大学农业部畜禽疫病诊断与免疫重点实验室,江苏南京210095

出　　处：《中国兽医学报》2006年第5期495-497,共3页Chinese Journal of Veterinary Science

基　　金：国家"973"项目子课题(G1999011906);上海市科技兴农重点攻关项目(农科攻字98第05-4号)

摘　　要：为获得控制猪链球菌病安全高效的疫苗,本试验进行了马链球菌兽疫亚种类M蛋白亚单位疫苗的研制。应用热酸法提取马链球菌兽疫亚种ATCC35246株类M蛋白,并分别采用羟基磷灰石(HAT)层析和冷酒精沉淀法进行纯化,结果得到较纯的类M蛋白。用热酸提取的粗制类M蛋白、2种纯化的类M蛋白及全菌苗免疫小鼠,结果保护率分别为92%(11/12)、100%(12/12)、100%(12/12)和83%(10/12),表明类M蛋白亚单位疫苗有进一步开发的必要。Streptococcus equi subsp, zooepidemicus,a lancifield group C Streptococcus,is an important pathogen of pigs. Previous studies have indicataed that M-like protein is an important virulence factor and protective antigen. We extracted the Mprotein by hot-acid ways ,and the acid-extracted proteins were partially purified by hydroxyapatite （HAT） chromatography and cold-alcohol precipitate. The whole cell bacterin,the acid-extracted protein and partially purified M-like protein were applied to vaccinate mice with the protective efficacy of 83.3% （10/12）,92% （11/12）, 100% （12/12） respectively. It suggested that the M-like protein vaccine against Streptococcus equi subsp. zooepidemicus would be necessary for to be further developed.

关 键 词：马链球菌兽疫亚种 类M蛋白 疫苗 

分 类 号：S852.4[农业科学—基础兽医学] S858.21[农业科学—兽医学]