猪生长激素抗独特型抗体的制备及免疫学鉴定  被引量:3

Preparation and Immunological Characterization of Anti-idiotypic Antibody for Porcine Growth Hormone

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作  者:郑鑫[1] 夏青娟[1] 朱世成[2] 郝林琳[1] 姜海龙[1] 宋海龙[1] 龙淼[1] 王哲[2] 

机构地区:[1]吉林农业大学动物科技学院,吉林长春130118 [2]吉林大学畜牧兽医学院,吉林长春130062

出  处:《中国兽医学报》2006年第5期516-518,共3页Chinese Journal of Veterinary Science

基  金:国家自然科学基金资助项目(30471265)

摘  要:应用猪生长激素(Ag)纯品,通过杂交瘤技术制备了猪生长激素单克隆抗体(Ab1)。Ab1经免疫亲和层析柱纯化后免疫新西兰白兔,其血清经饱和硫酸铵纯化后获得猪生长激素抗独特型抗体(Ab2)。再应用ELISA等方法进行鉴别。Ab2与Ab1呈阳性反应,与BALB/c小鼠r球蛋白呈阴性反应;Ab2与Ag竞争结合Ab1;Ab2抑制Ab1与Ag的结合。Ab1是针对猪生长激素纯品的单克隆抗体。Ab2是具有猪生长激素抗原内影像的抗独特型抗体。To produce anti-idiotypic antibody of porcine growth hormone and to identify it by immunological technique and cell culture experiment. Monoclonal antibody of porcine growth hormone (Ab1) was prepared by using the pure porcine growth hormone and B-lymphocyte hybridoma technology. Anti-idiotypic antibody of porcine growth hormone (Ab2) was produced by immunizing rabbits with Ab1 ,their serum was purified through SAS. Then it was identified through ELISA. The reaction of Ab2 was positive with Ab1 ,while it was negative with murine IgG through indirect ELISA test, Ab2 competitively bound Ab1 with Ag. Ab2 competitively inhibited the binding of Ab1 with Ag. Ab1 was monoclonal antibody of porcine growth hormone. Ab2 was an anti-idiotypic antibody bearing internal image of porcine growth hormone.

关 键 词:猪生长激素 抗独特型抗体 免疫 

分 类 号:S852.4[农业科学—基础兽医学] S858.28[农业科学—兽医学]

 

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