鸡白细胞介素-6活性MTT检测方法的建立  

Establishment of MTT Assay for Detecting Chicken Interleukin-6 Activity

作　　者：周全[1] 程国富[1] 胡薛英[1] 李秀梅[1] 周诗其[1] 谷长勤[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国兽医学报》2006年第5期522-524,共3页Chinese Journal of Veterinary Science

基　　金：湖北省自然科学基金资助项目(2003ABA100)

摘　　要：采用白细胞介素-6依赖细胞株B9建立了鸡白细胞介素-6活性的MTT检测方法。每孔培养细胞数在2．5×10^3～4×10^4范围内D值与细胞数显示有良好的线性关系，MTT的最佳保留时间为4h，最低检测限为0．1U／mL。应用该方法检测了健康艾维菌肉鸡25例，血清chIL-6活性为（4．33±0.75）U／mL，而25例葡萄球菌病患鸡血清chIL-6的活性为（14．05±6．87）U／mL，与健康肉鸡比较差异极显著（P〈0．01），为该方法进一步临床应用奠定了基础。A rapid,sensitive and simple method,using the IL-6 dependent cell line B9,and MTT colorimetric assay for determination of chIL-6 activity was established in the presen, experiment. A good linear relationship was found between the optical density value and the number of cells in each well when the number of cultured cells in each well ranged from 2.5× 10^3 to 4 × 10^4. The best incubation period with MTT is 4 h. And the detectable limit is 0.1 units（or 0. 1 pg） of IL-6 in one milliliter. The chIL-6 activity in serum of healthy broilers （n = 25） and broilers with staphylococcosis （n = 25） were measured. The values of average IL-6 activity were （4.33 ± 0.75） units/mL in healthy broilers, and （14.05 ± 6.87 ） units/mL in staphylococcosis broilers. There is significant difference between the two groups.

关 键 词：鸡白细胞介素-6 B9细胞株 MTT比色法 

分 类 号：S858.31[农业科学—临床兽医学] S852.4[农业科学—兽医学]