猪卵母细胞去核方法的改进  

A Modified Enucleation Method of Porcine Oocytes

作　　者：张德福[1,2] 刘东[1,2] 汤琳琳[1,2] 陈晓宇[3] Lin Cailu 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]西北农林科技大学动物科技学院,陕西杨凌712100 [4]Animal Breeding Institute,Bonn University,Bonn 53121 ,Germany

出　　处：《中国兽医学报》2006年第5期574-577,共4页Chinese Journal of Veterinary Science

基　　金：德国BMBF基金资助项目(CHN99/310);上海市科学技术委员会基金资助项目(014909005)

摘　　要：对猪体细胞克隆技术中关键步骤之一的去核方法作了较深入的研究。结果表明,由本实验室改进的Willadsen去核法———二步挤压去核法,无论在去核操作成功率(73.9%)上还是在去核效率(81.2%)上都明显好于McGrath-Solter去核法(42.5%、67.4%,P<0.05);同时,本试验还发现,卵母细胞成熟培养36 h后去核组去核效率(89.1%)显著地高于成熟培养44 h后去核组(55.8%,P<0.05),而核移植重构胚的发育率2个组间无显著差异。This experiment was conducted to study enucleated method, which is one of the key techniques for animal cloning. The results show that the modified Willadsen＇s method for enucleation ,two-step-squeezing method ,is significantly better than McGrath-Solter＇s method in the enucleated rates （73.9%,42.5%,P〈0.05） and the rates （81.2%,67.4%,P〈0.05） of absolutely removing nuclei matter. It was also found that the rates（89. 1%） of absolutely removing nuclei matter for 36 hmatured-in-vitro oocytes is significantly higher than that for 44 h（55.8% ,P〈0.05）.

关 键 词：猪 卵母细胞 去核 核移植 

分 类 号：S828[农业科学—畜牧学] S852[农业科学—畜牧兽医]