葛根素颈上神经节注射对快速老化痴呆小鼠学习记忆功能的影响  

Effect of puerarin injected into superior cervical sympathetic ganglia on learning and memory function of mice with dementia

作　　者：董文毅[1] 孙坚[2] 丁涛[1] 赵金茹[3] 夏淑杰[3] 林庶茹[3] 才丽平[3] 

机构地区：[1]中国医科大学附属第二医院中医科,辽宁沈阳110004 [2]中国医科大学附属第二医院实验动物中心,辽宁沈阳110004 [3]辽宁中医学院基础医学院分子生物学实验室

出　　处：《中国医科大学学报》2006年第3期235-236,共2页Journal of China Medical University

基　　金：辽宁省教育厅高等学校科研基金资助项目(20121267)

摘　　要：目的观察快速老化痴呆小鼠(SAM-P/8)经葛根素颈上神经节注射后学习记忆功能的改变,为老年性痴呆临床开展新的治疗手段提供实验依据。方法将50只SAM-P/8随机分为4组葛根素实验组、利多卡因对照组、生理盐水对照组、空白对照组。除空白对照组不进行颈上神经节注射之外,其余各组分别进行葛根素注射液、利多卡因注射液和生理盐水颈上神经节注射,并于实验后进行SAM-P/8的活动频度程度积分测定和水迷宫实验。结果葛根素实验组活动频度程度测定积分和达岸正确率均明显高于其他各组(P<0.05)。达岸时间和碰壁错误次数明显少于其他各组(P<0.01)。结论葛根素颈上神经节注射对SAM-P/8的学习记忆功能有明显的改善作用。Objective： To observe the changes of learning and memory function after puerarin was injected into superior cervical sympathetic ganglia of the senescence-accelerated mice （ SAM-P/8）, and thus to provide experimental basis for new clinical therapeutics of Alzheimer＇ s disease. Methods： Fifty SAM-P/8 mice were randomly divided into 4 groups ： puerarin treatment group, lidocaine control group, saline control group, and blank control group. Except those in blank control group, the mice in other 3 groups were injected with puerarin, lidocaine, and saline into superior cervical sympathetic ganglia, respectively. The scores of the frequency and extent of movement were determined. The water maze test was performed. Results：Compared with other 3 groups, the scores of the frequency and extent of movement and the accuracy of reaching platform were significandy higher in puerarin treatment group （ all P 〈 0.05 ） , and the time of reaching platform and the number of hitting against the wall were significantly lower （ all P 〈 0.01 ）. Conclusion： Puerarin injected into superior cervical sympathetic ganglia could improve the learning and memory function of the SAM-P/8 mice.

关 键 词：葛根素 颈上神经节 痴呆小鼠 快速老化 

分 类 号：R285.5[医药卫生—中药学]