大肠杆菌气管注入致急性肺损伤的实验研究  

Acute lung injury induced by intratracheal instillation of Escherichia coli in rabbits

作　　者：车玉虎[1] 刘志[1] 董雪松[1] 马涛[1] 

机构地区：[1]中国医科大学附属第一医院急诊科,辽宁沈阳110001

出　　处：《中国医科大学学报》2006年第3期262-264,共3页Journal of China Medical University

摘　　要：目的建立气管注入细菌致兔急性肺损伤(ALI)模型,为研究细菌致ALI的致病机制及其治疗提供理论依据。方法兔分为细菌注入组(Ⅰ组),生理盐水注入组(Ⅱ组),正常对照组(Ⅲ组)。对比观察3组以下指标血流动力学、呼吸力学、血气分析、支气管灌洗液中的细胞数、蛋白含量、肺组织湿重/干重比及病理检查。结果Ⅰ组与Ⅱ组、Ⅲ组比较,上述指标,均有明显差异(P均<0.05)。结论通过直接气管注入细菌可建立ALI模型。Objective： To establish the rabbit model of acute lung injury （ALI） by intratracheal instillation ofEscherlchia coli , and to study the pathogenesis of bacteria-induced ALI. Methods： Nineteen New Zealand rabbits were divided into 3 groups ： group 1 （ intratracheally instilled Escherichia coli ）, group 2 （ intratracheally instilled sallne）, and group 3 （ control group）. The indexes, including hemodynamics, respiratory mechanics, blood-gas analysis, cell count in bronchial lavage fluid, protein content, lung wet-to-dry weight ratio, and pathological examination, were determined in these 3 groups. Results：There were significant differences in the above indexes both between group 1 and group 2 and between group 1 and group 3 （ both P 〈 0.05）. Conclusion： ALI model can be established by intratracheal instillation of Escherichia coli.

关 键 词：急性肺损伤 气管动物模型 

分 类 号：R563.8[医药卫生—呼吸系统]