葛根素注射液对急性酒精中毒大鼠β-内啡肽、丙二醛、P-选择素影响的实验研究  

Effect of puerarin on β-endorphin, malonyldialdehyde, and P-selectin in rats with acute alcohol poisoning

作　　者：杜艳秋[1] 赵敏[1] 李长喻[1] 

机构地区：[1]中国医科大学附属第二医院急诊科,辽宁沈阳110004

出　　处：《中国医科大学学报》2006年第3期269-270,共2页Journal of China Medical University

摘　　要：目的研究急性酒精中毒时大鼠β-内啡肽(β-EP)、丙二醛(MDA)、P-选择素(P-selectin)的变化及葛根素注射液的作用。方法将大鼠分为对照组、酒精中毒组、葛根素组、纳络酮组、葛根素+纳络酮组;观察大鼠睡眠潜伏时间,测定各组血浆β-EP、血清MDA、P-selectin水平。结果与对照组比较,酒精中毒组大鼠β-EP、MDA、P-selectin水平显著升高(P<0.01);葛根素组较酒精中毒组大鼠睡眠的潜伏时间延长(P<0.05),β-EP、MDA、P-selectin水平显著降低;葛根素与纳洛酮降低β-EP、MDA作用无显著差异,纳洛酮对P-selectin无影响。结论急性酒精中毒可使β-EP释放增加,MDA、P-selectin升高。葛根素注射液通过抑制β-EP、自由基的释放、血小板积聚,对急性酒精中毒大鼠起保护作用。Objective： To investigate the changes of β-endorphin （β-EP）, malonyldialdehyde （MDA）, and P-selectin and the protective effect of puerarin in rats with acute alcohol poisoning. Methods： Forty Wistar rats were divided into 5 groups ： control group, alcohol poisoning group, puerarin group, naloxone group, and puerarin plus naloxone group. The latent period of sleep was measured, and the levels of β-EP, MDA, and P-selectin were detected. Results： The levels of β-EP, MDA, and P-selectin in alcohol poisoning group were significantly higher than those in control group （ P 〈0.01 ）. The latent period of sleep in puerarin group was significantly longer than that in alcohol poisoning group （ P 〈 0.05）. There was no significant difference in the levels of β-EP and MDA between puerarin group and naloxone group. Naloxone had no effect on the level of P-selectin. Conduslon： The levels of β-EP, MDA, and P- selectin increases in rats with acute alcohol poisoning. Puerarin may have a protective effect on acute alcohol poisoning by inhibiting the production of β-endorphin, the release of free radicals, and platelet aggregation.

关 键 词：急性酒精中毒 Β-内啡肽 丙二醛 P-选择素 葛根素 

分 类 号：R595.6[医药卫生—内科学]