测定植物提取液中生物碱含量的间接萃取比色法研究  

A Research of Indirect Extraction-Spectroophotometric Mathod for Determinating the Content of Alkaloid in the Extract of Plants

作　　者：黄薇[1] 陈颖[1] 王淑英[1] 

机构地区：[1]山东枣庄师专化学系,首都师范大学化学系

出　　处：《淮北煤师院学报（自然科学版）》1996年第4期65-68,共4页Journal of Huaibei Teachers College(Natural Sciences Edition)

摘　　要：本文报告了在非离子表面活性剂存在下，用溴甲酚绿离子缔合萃取光度法测定植物提取液中生物碱的总量．比较了五种提取方法，探讨了测定的优化条件，确定用水饱和的正丁醇溶液提取效果最佳．测得一些植物的生物碱含量约０．１－０．９％．加标回收率９２％，相对标准偏差７％，结果令人满意．In this paper,it is reported that with the existence of nonionic surfactants the total amount of alkaloid in the extract of plants is determinedby extraction-spectrophotometric method of bromocresol green ion-association.Five extracting methods are compared to explore the optimum condition,and the water-saturated n-butanol is warranted to be the best extracting agent.The content of alkaloid in the extract of several plants is about 0.1￣0.9%,the rate of recovery of the added satndard liquid is over 92%,relative standard deviation is about 7% (n=5),The results are satisfactory.

关 键 词：生物碱 萃取光度法 植物 提取液 测定 

分 类 号：Q946.88[生物学—植物学]