春砂仁多糖的提取及组分分析  

Purification and analysis of the Amomum villosum Lour.polysaccharides

作　　者：樊亚鸣[1] 黄晓兰[2] 陈永亨[1] 廖子英[1] 林海清[1] 

机构地区：[1]广州大学化学与化工学院,广东广州510006 [2]中国广州分析测试中心,广东广州510070

出　　处：《广州大学学报（自然科学版）》2006年第4期30-32,共3页Journal of Guangzhou University:Natural Science Edition

基　　金：国家星火计划项目(2005EA780029)

摘　　要：报道春砂仁多糖的分离纯化及结构分析的研究.以凝胶过滤色谱法测定春砂仁多糖的数量及分子量,结果显示:春砂仁主要含两种多糖,其数均分子量分别为56 421及1 743;多糖经酸解成为单糖并衍生为乙酰化的糖肟,对照标样采用气相色谱法测定其单糖组分为阿拉伯糖、甘露糖、葡萄糖及半乳糖,4种单糖含量的相对比例:1.00∶0.68∶0.97∶0.86.The paper reported the purification and analysis of the Amomum villosum Lour. polysaccharides. The number and molecular weight of the Amomum villosum Lour. polysaccharides were tested by Gel Filtration Chromatography （GFC） , which showed that there are two kinds of polysaccharides in Amomum villosum Lour. and that their Mn are 56 421 and 1 743, respectively. After the polysaccharides were hydrolyzed in acidic condition, the corresponding monosaccharides formed and were transformed into acetyl derivatives by acetic anhydride. The derivatives were tested by Gas chromatography （GC） to give the result that the four monosaccharides exist with the certain proportion： arabinose： mannose： glucose： galactose = 1.00： 0.68： 0.97： 0.86.

关 键 词：春砂仁多糖 多糖分子量 多糖数量 单糖组分 

分 类 号：R281.5[医药卫生—中药学]