二氢埃托啡处理对大鼠脑区μ受体mRNA水平的调节  

Effects of dihydroetorphine treatment on regulation of μ opioid receptor mRNA level in rat brain regions

作　　者：王丹心[1] 秦伯益[1] 

机构地区：[1]军事医学科学院毒物药物研究所

出　　处：《中国药理学与毒理学杂志》1996年第4期247-250,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：采用液相杂交技术，对二氢埃托啡（ＤＨＥ）和吗啡（Ｍｏｒ）处理大鼠的中脑，海马和纹状体中μ受体ｍＲＮＡ含量进行测定．大鼠皮下注射ＤＨＥ３ｄ或Ｍｏｒ１４ｄ可分别对ＤＨＥ或Ｍｏｒ产生耐受．中脑μ受体ｍＲＮＡ水平在ＤＨＥ或Ｍｏｒ处理３ｄ即下降，海马和纹状体中μ受体ｍＲＮＡ在处理７ｄ或２１ｄ后亦开始下降．表明活体ＤＨＥ或Ｍｏｒ处理可引起大鼠不同脑区μ受体ｍＲＮＡ下调，这种下调可能与ＤＨＥ或Ｍｏｒ的耐受有关，同时耐受的产生可能还有其它机理．The μ opioid receptor mRNA levels in rat midbrain, hippocampus and striatum after dihydroetor- phine(DHE) and morphine (Mor) treatment were tested by solution hybridization. Rats rendered tolerance to DHE or Mor after 3 d of DHE or 14 d of Mor treatment. Whereas, the μ receptor mRNA level was decreased in midbrain after only 3 d of DHE or Mor treatment. And the μ receptor mRNA levels in hippocampus and in striatum were also decreased after 7 and 21 d of DHE or Mor treatment respectively. These results indicate that down-regulation of μ opioid receptor mRNA can occur in vivo in rat brain regions after DHE or Mor treatment and this down-regulation of μ receptor may be correlated to DHE or Mor tolerance. However, in addition to μ receptor down-regulation, other mechanisms may also be involved in tolerance.

关 键 词：脑区 Μ受体 二氢埃托啡 MRNA 

分 类 号：R971.1[医药卫生—药品]