生殖器疱疹病毒抗原的两种检测结果比较分析  

Comparison of two methods in detection of genital herpes virus

作　　者：张帆[1] 刘芳[2] 邓俐[1] 于磊[1] 郑俊炜[1] 张堂德[1] 黄亦加[1] 莫银娟[1] 

机构地区：[1]南方医科大学珠江医院皮肤科,广州510282 [2]南方医科大学珠江医院门诊部,广州510282

出　　处：《微生物学免疫学进展》2006年第3期27-28,共2页Progress In Microbiology and Immunology

摘　　要：比较分析生殖器疱疹病毒抗原的两种检测方法,找出更为准确、简便、经济、实用的检测方法,分别利用疱疹病毒抗原酶免疫(ELISA)法和荧光定量PCR(FQ-PCR)法检测南方医科大学珠江医院门诊患者538例。酶免疫法检出率为27.5%,略低于荧光定量PCR法的28.3%,经SPSS12.0分析,两种方法检测结果无显著差异。酶免疫法检测生殖器疱疹病毒抗原具有操作简便、快捷、经济等特点,而敏感性和特异性均无显著差别,是一种更适合临床的检验方法,更具有值得推广使用的价值。Search for a diagnostic method was conducted, with a higher sensitivity and reliability including economical and practical advantages in detection of genital herpes virus （ HSV-2 ）. ELISA and FQ-PCR were used to detect 538 clinical specimens in our hospital, in comparison with the two detection methods. The results showed 148 of them were ELISA positive and 152 of them were FQ-PCR positive, on which had no significant difference in between two methods by using software SPSS 12.0. It suggest that ELISA is suitable to be used as a common laboratory diagnostic method for HSV-2.

关 键 词：生殖器疱疹 ELISA FQ—PCR 

分 类 号：R446.6[医药卫生—诊断学]