猪UCP3基因的分离与多态分析  

The isolation of uncoupling protein-3 gene of pigs and analysis of its polymorphism

作　　者：朱宝芹[1] 任亮[1] 苏玉虹[1] 

机构地区：[1]锦州医学院辽宁省分子细胞生物学与新药开发重点实验室,辽宁锦州121000

出　　处：《畜牧与兽医》2006年第6期14-17,共4页Animal Husbandry & Veterinary Medicine

基　　金：辽宁省科技厅博士启动基金(20021072);辽宁省教育厅基金(2004F112)

摘　　要：解偶联蛋白3是一种线粒体内膜上的转运蛋白，呵以使氧化过程与ADP磷酸化过程解偶联。本研究分离了猪UCP3基因的外显子2～3和外显子6。经过序列分析，发现猪UCP3基因共有3处框移突变和18个SNP位点。在猪UCP3片段中分别包含一个SmaⅠ和SduⅠ酶切位点。UCP3片段SmaⅠ位点在大白×梅山F2家系中经PCR—RFLP分析，表现为AA、AB和BB3种基因型，A基因频率为0．57，B基因频率为0．43。因此，可以将UCP3基因作为猪分子遗传育种的候选基因。Uncoupling protein 3 （UCP3） was mitochondrial membrane transporters involved in themogenesis, which uncoupled the oxida- tion and phosphorylation of ADP on respiration chain. Exon 2 - 3 and 6 of UCP3 gene were isolated in this study. By DNA sequences analysis, three frame-shift mutation and eighteen SNP were found in UCP3 gene of pigs. There were restriction enzyme sites of Sma Ⅰand Sdu Ⅰ in UCP3 gene of pigs. The characterization of the Sma Ⅰ polymorphism in UCP3 gene was analyzed by PCR-RFLP among ＂Largewhite x Meisban＂ F2 group. The genotypes of UCP3 were AA, AB and BB. The frequencies of A allele is 0. 57 and B is 0.43.

关 键 词：猪 解偶联蛋白3 PCR—RFLP 遗传效应分析 

分 类 号：S828.2[农业科学—畜牧学]