荔枝体胚发生过程中的细胞学观察  

Cytological observation of somatic embryogenesis in litchi

作　　者：邓朝军[1] 易干军[2] 曾继吾[2] 张秋明[3] 

机构地区：[1]福建省农业科学院果树研究所,福州350013 [2]广东省农业科学院果树研究所,广州510640 [3]湖南农业大学园艺园林学院,长沙410128

出　　处：《果树学报》2006年第5期760-762,共3页Journal of Fruit Science

基　　金：广东农业科学院院长基金项目(04-基金-04B)。

摘　　要：以荔枝三月红胚性愈伤组织为材料,采用改良的石蜡切片法观察荔枝胚性愈伤组织结构及体胚发生过程,结果显示荔枝的体胚发生经历球形、心形、鱼雷形、子叶形4个过程;荔枝在胚性细胞分裂发育形成体胚之前,出现与周围细胞明显不同的界限,形成细胞间隔离;比较荔枝胚性愈伤组织与非胚性愈伤组织的细胞学特性,发现胚性愈伤组织具有核大、质浓、染色深、细胞排列紧密等特点,与非胚性愈伤组织明显不同。通过系统观察荔枝胚性愈伤组织结构以及胚性细胞分裂、生长方式和体胚发生过程,为胚性愈伤组织的利用提供细胞组织学依据。Observation was conducted with Sanyuehong litchi cuhivar through paraffin sections. The observation on the configuration of the embryogenic callus and the process of somatic embryo growth found that the embryoids were sequentially differentiated through embryonic suspensor masses, globular, heart-shaped, torpedo embryo and cotyledonary embryo stages. Before embryogenesis, the embryogenic callus had formed evident circumscription. Compared with the non-embryogenie callus, the cell of the embryogenic callus possessed denser protoplasm and larger nucleus. Observing the configuration of embryogenic callus, the manner of embryogenic cell division and the process of somatic embryo growth can provide the cytological and histological basis for using embryogenic callus in litchi.

关 键 词：荔枝 体胚发生 细胞学 愈伤组织 

分 类 号：S667.1[农业科学—果树学]