生物法净化苯、甲苯、二甲苯废气菌种驯化  

Acclimation of High Biodegradability Consortium of Benzene Toluene and Xylene in a Biotrickling Filter

作　　者：王丽萍[1] 华素兰[1] 赵跃民[1] 朱玉丽[1] 徐龙乾[1] 

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221008

出　　处：《中国矿业大学学报》2006年第5期633-636,641,共5页Journal of China University of Mining & Technology

基　　金：江苏省自然科学基金项目(BK2004031);江苏省环境科技发展基金项目(2004007);中国矿业大学科学研究基金项目(0P4069)

摘　　要：对生物滴滤器净化苯、甲苯、二甲苯废气菌种驯化进行了研究，创建了“诱导物-目标污染物”专性降解菌双底物驯化模式，以甲苯为诱导物，进行了“甲苯-苯”、“甲苯-二甲苯”双底物驯化，并将苯专性降解菌接种于Ф40mm×500mm的生物滴滤器进行净化笨的工艺实验．结果表明：“甲苯-苯”驯化第25天时，其假单胞杆菌密度高达2．08×10^8CFU／mL，比苯直接驯化高出约5×10^7CFU／mL；“甲苯-二甲苯”驯化第25天时，其假单胞杆菌密度为1．60×10^8CFU／mL；净化苯试验表明，气量为60L／h，苯的质量浓度为0．747～3．760mg／L条件下，苯平均去除能力高于120g／（m^3·h），峰值去除能力达到246g／（m^3·h）（此时假单胞杆菌密度高达6．0×10^10CFU／mL），采用双底物驯化方式优于直接驯化．In order to develop the effective controlling technology for emission of Benzene, Toluene and Xylene （BTX）, the microflora acclimation for the purification of BTX was studied. A novel acclimation method, called inducer-target graded acclimation, was proposed, in which the toluene was used as the inducer substrate, the benzene and xylene were target ones. The toluene-benzene and toluene-xylene dual substrates were employed to acclimate the benzene and xylene biodegradation consortium. The benzene-removal experiment was performed on the bench- scale biofilter trickling system of Ф40 mm× 500 mm for validating this acclimation method. The results of Benzene-removal experiments show that when the gas flux of 60 L/h and benzene concentration in inlet gas of 0. 747-3.760 mg/L, the maximum biological elimination capacity of benzene in waste gas of 246 g/（m^3 · h） and the steady biological elimination capacity of over 120 g/（m^3 · h） were achieved. The Pseudomonas putida is the dominant bacterial and the density amounts to 6.0 × 10^10 CFU/mL according to the observation for biotic community.

关 键 词：生物滴滤器 BTX苯 双底物驯化 去除能力 

分 类 号：X701[环境科学与工程—环境工程]