电刺激诱导大鼠海马癫痫电网络神经信息分析  

AN ANALYSIS OF NEURAL INFORMATION WITHIN ELECTROGENIC HIPPOCAMPAL EPILEPTIC NETWORK IN RATS

作　　者：吴俊芳[1] 王文挺[1] 魏晓菲[1] 韩丹[1] 

机构地区：[1]武汉大学基础医学院生理学系,武汉430071

出　　处：《生物物理学报》2006年第4期259-267,共9页Acta Biophysica Sinica

基　　金：湖北省自然科学基金项目(2003ABA123)~~

摘　　要：探讨电刺激致海马(hippocampus,HPC)癫痫网络的神经信息特征和M型胆碱能受体阻断剂东莨菪碱(scopolamine)对该信息特征的调制作用。实验用雄性SD大鼠45只,体重150￣250g。急性强直电(60Hz,2s,0.4￣0.6mA)刺激右侧后背HPC(acutetetanizationoftherightposteriordorsalhippocampus,ATPDH),双电极同步记录同侧HPC网络和单个神经元电活动。分析癫痫发作样高频电振荡(ripple)功率谱(powerspec-trum)、尖波连续发放峰间间隔(interpeakinterval,IPI)和单位时间内平均频率(Hz),并同步分析单个神经元放电脉冲间隔(interspikeinterval,ISI)的变化特征。发现:(1)ATPDH诱导的HPC癫痫放电模式主要包括rip-ple和具有稳定频率特征的尖波样连续发放;(2)东莨菪碱(i.p.)可以提前ripple第1组分最大功率(μV2)与单个神经元原发性单位后放电最大ISI出现的时间,对最大ISI的作用更明显;(3)东莨菪碱可以部分再现重复施加ATPDH诱导出现巨大尖波连续发放IPI和神经元放电ISI平行发展特征。结果提示:M胆碱能受体阻断剂东莨菪碱可以同时调制HPC癫痫网络成员电场和细胞的瞬时编码信息;而成员电场ripple功率谱/连续尖波IPI和神经元放电ISI点分布的对比研究,可以用于分析癫痫网络瞬时编码信息和药物生物学效应。The present work was to detect the modulation of scopolamine on characteristics of neural information of electrogenic hippocampal epileptic network in rats. Experiments were performed on 45 SD male rats, weighting 150-250 g. The hippocampal epilepsy was induced by the acute tetanization （60 Hz, 2 s, 0.4-0.6 mA） of the right dorsal hippocampus （ATPDH）. The ipsilateral hippocampal field potentials and single unit discharges were simultaneously recorded. The power spectrum, power/time of the hippocampal ripples （up to 150 Hz）, the interpeak intervals （IPI）, of continual robust sharp waves, and the interspike intervals （ISI） of single neuronal firing were analyzed for extracting temporal code information of hippocampal epileptic network. The result demonstrated： （1） Ripples and continual sharp waves were evoked by the repetitive ATPDH; （2） Scopolamine （0.05 mg/kg, i.p.） shortened the occurrence time of the maximal power spectra （μV2） during the first ripple component and of the maximal ISI of single neuronal firing, but remarkably shortened the latter; （3） and neuronal firing ISI reappeared partially after the A parallel development of robust sharp wave IPI injection of scopolamine, which was induced by repetitive ATPDH trains. It suggested that the temporal coding information of the member fields and neurons could be modulated by the scopolamine simultaneously within hippocampal epileptic network. Both the ripple power spectra and the IPI scatter of hippocampal network could be matched with single neuronal firing ISI scatter for extracting temporal code information.

关 键 词：海马癫痫 功率谱 峰间隔 放电脉冲间隔 电刺激 大鼠 

分 类 号：Q424[生物学—神经生物学] R742.1[生物学—生理学]