免疫磁珠法分离纯化人牙周膜干细胞  

Isolation purification of human periodontal ligament stem cells using immunom agnetic beads

作　　者：高秦[1] 刘宏伟[2] 金岩[3] 

机构地区：[1]解放军第1医院口腔科,甘肃兰州730030 [2]上海同济大学口腔医学院 [3]第四军医大学口腔医院病理科

出　　处：《临床口腔医学杂志》2006年第9期520-522,共3页Journal of Clinical Stomatology

基　　金：陕西省自然科学研究计划(2003C2042)

摘　　要：目的：建立免疫磁珠法体外分离纯化人牙周膜干细胞的方法。方法：采用STRO-1作为磁珠分离细胞的表面标志,利用间接法免疫磁珠分离细胞,描绘其生长曲线及免疫组化染色检测抗波形丝蛋白(Vimentin)、骨粘连素(ON)、骨桥蛋白(BSP)的表达。结果：通过免疫磁珠法获得了人牙周膜STRO-1^+细胞,观察其生长曲线表明其为慢周期性；该细胞抗波形丝蛋白(Vimentin)、骨粘连素(ON)、骨桥蛋白(BSP)弱阳性表达。结论：免疫磁珠法是有效分离纯化牙周膜干细胞的方法,该方法分离的人牙周膜STRO-1^+细胞具有干细胞的超微结构、细胞周期及表型特点。Objective： To isolate and identify the periodontal ligament stem cells from human molars. Method： Periodontal ligament cells were incubated with antibody of STRO-1 and these cells were sperated from the suspension through the magnetic field by immunom agnetic beads coated with the second antibody , and immunohistochemistry was performed to inderltify surface marker of the STRO-1^＋ cells. Result： The cells showed the characteristics of undifferentiated cells at the ultrastructural level and they positively expressed surface marker of Vimentin, ON and BSP. Conclusion： immunom agnetic beads may be the effective way to isolate and purify the periodontal ligament stem cells and the isolated cells were self-renewal, undifferentiated, quiescent dental mesenchymal originated.

关 键 词：人牙周膜干细胞 免疫磁珠 免疫组织化学 

分 类 号：Q813.1[生物学—生物工程]