不同物种输卵管上皮细胞培养、纯度检测及支持小鼠胚胎发育的能力  

Culture,Purification and Ability to Support Mouse Embryo Development of Oviductal Epithelial Cells from Different Species

作　　者：谭秀文[1] 马所峰[1] 刘新勇[1] 张霞[1] 兰国成[1] 于建宁[1] 张俊功[2] 谭景和[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省农业科学院畜牧兽医研究所,济南250100

出　　处：《畜牧兽医学报》2006年第9期878-882,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金资助项目(30170679;30430530;30571337);国家"973"计划项目(G200016108)

摘　　要：针对不同物种采用不同方法培养小鼠、山羊和鸡输卵管上皮细胞(OECs)并检测上皮细胞纯度,在此基础上比较了它们支持小鼠胚胎发育的能力。结果表明,3种细胞都能很好地生长,培养的细胞几乎全为上皮细胞(Vi-mentin单克隆抗体检测显示,小鼠、山羊和鸡OECs的阳性细胞率分别为2.4±0.3、1.3±0.3和1.8±0.4)。昆明白小鼠原核胚与小鼠、山羊和鸡OECs共培养,发育到囊胚的比例分别为61.7%、57.2%和56.2%,差异不显著(P>0.05)。说明鸡和山羊OECs能很好地支持小鼠胚胎发育。Oviductal epithelial cells （OECs） of mouse , goat and chick were cultured by different methods and examined percents of Vimentin positive cells by Vimentin monoclonal antibody, then on this basis in vitro development of mouse pronuclear embryos co-cultured with them were compared. The results are summarized as follows： Somatic cells grow well in culture system and almost all are epithelial cells. Percents of Vimentin positive cells of mouse , goat and chick OECs were 2.4±0.3, 1.3 ± 0.3 and 1.8 ± 0.4, respectively. The proportion of pronuclear embryos from Kunming mouse developing to blastocysts （61.7% ,57.2% and 56.2%, respectively） did not differ when co-cultured with primary mouse, goat and chick OECs, suggesting that a good development of mouse embryos was also achieved when co-cultured with goat and chick OECs.

关 键 词：胚胎 体细胞 共培养 小鼠 

分 类 号：Q813.7[生物学—生物工程]