人参皂甙Rb1和黄芪多糖对微血管内皮细胞分泌NO、IL-6和TNF-α的影响  

Effect of Ginsenoside Rb1 and Astragalus Polysaccharide on the Excretion of NO,IL-6 and TNF-α in Micro-vascular Endothelial Cells

作　　者：解慧梅[1] 胡格[2] 索占伟[2] 刘易通[1] 尹龙[2] 于同泉[2] 路平[2] 穆祥[2] 黄会岭[1] 

机构地区：[1]河北农业大学动物科技学院,保定071001 [2]北京农学院动物科技系,北京102206

出　　处：《畜牧兽医学报》2006年第9期903-907,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业应用新技术北京市重点实验室(KF2003-1)

摘　　要：取中药提取物人参皂甙Rb1和黄芪多糖作用于体外培养大鼠肠黏膜微血管内皮细胞,用硝酸还原酶法测定一氧化氮(NO)、ELISA法测定白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平的变化,为分析其免疫作用机制提供试验依据。微血管内皮细胞在人参皂甙Rb1和黄芪多糖的作用下,细胞分泌NOI、L-6和TNF-α均增多,并且随着浓度的增加内皮细胞分泌量也增加。Two Chinese herbal medicine extraction, ginsenoside Rb1 and astragalus polysaccharide, were supplemented into in vitro cluture system of rat micro-vascular endothelial cells. NO was detected by reduction of nitrate method and IL-6,TNF-α were detected by ELISA, the changes of those relevant factors provided experimental evidence for analyzing effect of ginsenoside Rbl and astragalus polysaccharide. The level of NO,IL-6 and TNF-α in micro-vascular endothelial cells were enhanced significantly by ginsenoside Rbl and astragalus polysaccharide, and showed concentration-dependent trend.

关 键 词：黄芪多糖 人参皂甙RB1 微血管内皮细胞 NO IL-6 TNF—α 

分 类 号：S853.7[农业科学—临床兽医学]