大豆遗传图谱的构建和抗胞囊线虫(Heterodera glycines Ichinohe)的QTL分析  

Construction of A Soybean Genetic Linkage Map and Mapping QTLs Resistant to Soybean Cyst Nematode (Heterodera glycines Ichinohe)

作　　者：卢为国[1] 盖钧镒[1] 郑永战[1] 李卫东[2] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,江苏南京210095 [2]河南省农业科学院棉花油料作物研究所,河南郑州450002

出　　处：《作物学报》2006年第9期1272-1279,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金资助项目(30490250);国家重点基础研究发展规划项目(973计划)项目(2002CB111304;2004CB7206);国家高技术研究发展计划(863计划)项目(2002AA211052);河南省自然科学基金项目(0211030800);长江学者和创新团队发展计划资助项目

摘　　要：大豆胞囊线虫1号和4号生理小种是黄淮地区的优势小种，ZDD2315是我国特优抗源。本文旨在定位ZDD2315对1号和4号生理小种抗性的QTL。试验以Essex为母本，ZDD2315为父本和轮回亲本，创建了一个包含114个单株的Bc。群体。采用250个SSR标记和1个形态标记通过MAPMAKER3．0构建了包含25个连锁群的遗传图谱，覆盖大豆基因组2963．5cM，平均每个连锁群上10．0个标记，标记平均间距11．8cM。采用Win QTL Cartographer Version 2．5复合区间作图法（CIM）检测到3个抗1号小种的QTL；其中rhgR1-1和rhgR1—2位于G连锁群的Sat_210～Sat_168和Sat_168～Sat_141区间，贡献率分别为22．4％和21.8％；rhgR1-3位于D2连锁群的Satt672～Satt413区间，贡献率6．2％；rhgR1-1和rhgR1—3分别与Sat_210和Satt672共分离。5个QTL与抗4号生理小种有关；其中rhgR4—1和rhgR4—-位于G连锁群的Satt275～Sat_210和Sat_168～Sat_141区间，贡献率分别为22．8％和28．9％；rhgR4—3和rhgR4—4位于H连锁群Satt442～Sat401和Sat_334～Satt181区间，贡献率分别为12．0％和10．5％；rhgR4—5位于L连锁群Satt652～Sat_301区间，贡献率5．9％；吨职4—2和rhgR4—5分别与Sat_168和Satt652共分离。不同遗传体系控制ZDD2315对1号和4号小种的抗性。抗1号和4号生理小种的主要QTL位于G连锁群的相近区段，且具有较大贡献率，通过标记辅助选择有可能育成兼抗两小种的品种。Race 1 and Race 4 are predominant races of soybean cyst nematode （SCN） in Huang-Huai Valleys in China. The soybean land race, ZDD2315, was recognized as an elite resistance source for the races. The present study was aimed at mapping QTLs conferring resistance to Race 1 and 4 in ZDD2315. The mapping population with 114 BC1F1 plants of the backcross （Essex × ZDD2315） × ZDD2315 was established and used to construct a genetic linkage map by using 250 SSR markers plus one morphological marker under MAPMAKER 3.0, spanning 25 linkage groups, each with 2 to 20 markers, at a total length of 2 963.5 cM, and average marker distance of about 11.8 cM. The software Win QTL Cartographer Version 2.5 （CIM） was used for QTL mapping of the data. Three QTLs conferring resistance to SCN Race 1 were mapped, among which rhgR1-1 and rhgR1-2 located in Sat_ 210-Sat_ 168 and Sat_ 168-Sat_ 141 on linkage group G and rhgR1-3 located in Satt672 - Satt413 on linkage group D2, explaining 22.4%, 21.8% and 6.2% of the total phenotypic variation, while rhgR1-1 and rhgR1-3 co-segregated with Sat_ 210 and Satt672, respectively. Five QTLs conferring resistance to SCN race 4 were mapped, among which rhgR4-1 and rhgR4-2 in Satt275-Sat_ 210 and Sat 168-Sat_ 141 on linkage group G, rhgR4-3 and rhgR4-4 in Satt442-Sat_ 401 and Sat_ 334-SattlS1 on linkage group H, and rhgR4-5 in Satt652-Sat_ 301 on linkage group L, explaining 22.8%, 25.9%, 12.0%, 10.5% and 5.9% of the total phenotypic variation, while rhgRd-2 and rhgRd-5 co-segregated with Sat_ 168 and Satt652, respectively. Therefore, different QTL systems conferred resistance to Race 1 and Race 4 in ZDD2315. Since the QTLs resistant to Race 1 and Race 4 located on linkage group G explaining a large part of phenotypic variation, it is possible to develop cultivars resistant to both races through marker-assisted selection.

关 键 词：大豆 遗传图谱 胞囊线虫(SCN) QTL定位 

分 类 号：S565.1[农业科学—作物学]