棉属种间杂交基因渐渗系SSR标记及其表型性状的聚类分析  

Cluster Analysis of the Introgressed Lines from Interspecific Hybridization in Cotton based on SSR Markers and Phenotype Traits

作　　者：庞朝友[1] 杜雄明[1] 马峙英[2] 

机构地区：[1]中国农业科学院棉花研究所,河南安阳455112 [2]河北农业大学,河北保定071001

出　　处：《作物学报》2006年第9期1371-1378,共8页Acta Agronomica Sinica

基　　金：国家"十五"重点科技攻关计划项目(2004BA525B05)

摘　　要：利用37对多态性SSR引物和15个表型性状对155份棉属种间杂交基因渐渗系及其10份陆地棉亲本进行了聚类分析。用37对SSR引物共扩增出108条带，多态性条带占85．2％。种质间成对相似系数平均值为0．6791，范围为0．4762～1．000。建立了165个陆地棉材料的SSR聚类图，共聚为4个类群，分别为法国种质群、美国AcalaSJ系列种质群、美国PD种质群和中国种质群，聚类结果与种质材料的系谱来源基本吻合。表型性状数据分析表明，各种质间的欧氏距离为0．6417～10．3075，平均5．0047，基于表型性状，将155份渐渗系聚为9个类群，类群问性状表现差异较大。表型性状聚类结果与材料系谱来源相莘悬殊。综合利用SSR、表型性状聚类结果将促进棉属种间杂交基因渐渗材料的高效利用。The main cultivated varieties belonged to the species of Upland cotton （ Gossypium hirsutum L. ） in the world and their genetic background is very narrow. However, The wild species and race in the genus of Gossypium have abundant genetic polymorphisms and possess lots of excellent genes with potential high yield, fine and strength fiber, disease and insect resistance, drought and coldness resistance, male sterility and so on. The interspecific hybridization for utilizing the benefit genes from these wild species had been widely carried out for 15 years in China. It has huge practical and theoretical value to conduct the analyses of the cotton introgression lines of interspecific hybridization toward upland cotton, which help to increase the genetic basis of the present upland cotton resources and break the bottleneck of cotton breeding. In this paper, the cluster analysis of 155 introgressed lines from interspecific hybridization and 10 parents of Gossypium hirsutum was conducted by using 37 pairs of polymorphic SSR primers and 15 phenotype traits （ Table 1 ） . Based on 37 pairs of polymorphic SSR primers, 108 bands altogether were amplified and the percent of polymorphic bands was 85.2 %. Average paired similarity coefficient among germplasm was 0.6791, ranged from 0.4762 to 1.000. The SSR cluster dendrogram of 165 varieties of Gossypium hirsutum was constructed （Fig.2） with 4 groups： the French group, the American AcalaSJ series, the American PD lines and the germplasm in China. The result of SSR cluster analysis fitted basically with that of their pedigrees. The Euclidean distance was 0.6417-10. 3075 among different germplasm with an average of 5.0047. The 155 introgressed lines were divided into 9 groups by cluster analysis of their phenotypes （Fig. 3） . The agronomic and economic characters in different groups were significantly different. There was an obvious difference between the cluster analysis of phenotype traits and the pedigrees of these germplasm. Comprehensive utilization of SSR a

关 键 词：渐渗系 棉属种间杂交 遗传多样性 表型性状 SSR 

分 类 号：S562[农业科学—作物学]