内毒素致新生大鼠急性肺损伤IL-18的变化  

Plasma and lung tissue IL-18 change in endotoxin induced acute lung injury of neonatal rat

作　　者：王崇伟[1] 程锐[1] 秦勤[1] 蒋小镛[1] 许植之[1] 周晓玉[1] 

机构地区：[1]南京医科大学附属南京市儿童医院,210008

出　　处：《中国新生儿科杂志》2006年第4期210-212,共3页Chinese Journal of Neonatology

摘　　要：目的探讨内毒素致新生大鼠急性肺损伤与IL-18水平的关系。方法内毒素5mg/kg腹腔注射7日龄SD新生大鼠致急性肺损伤模型,同时设腹腔内注射生理盐水对照组,每组12只。注射后1、4、8h采集血,1、4h采集肺组织,检测血浆,肺组织匀浆IL-18水平。结果内毒素注射后新生大鼠1h,血浆IL18水平就显著上升(6.38±1.62)pg/ml,4h达到高峰(34.28±17.89)pg/ml,8h后明显回落(14.61±2.16)pg/ml,仍高于生理盐水对照组(1.63±1.18)pg/ml;4h组肺组织匀浆IL-18水平(508.96±105.38)pg/ml显著高于1h组(346.08±21.23)pg/ml(P<0.01),且肺组织匀浆IL-18水平高于同时点血浆IL18水平(P<0.01)。结论IL18是一种早期的致炎因子,在急性肺损伤的发生中起重要的作用,可作为急性肺损伤的诊治指标之一。Objective To investigate pro-inflammatory interleukin-18 （IL-18） change and its relation to lipopolysaccharide （LPS） induced acute lung injury （ALI） of neonatal rat. Methods ALI animal model was made by intraperitoneal injection of LPS 5 mg/kg. Enzyme-linked immunosorbent assay （ELISA） was used to determine plasma or lung tissue IL-18 level in neonatal rats 1, 4 and 8 h after injury. Results The plasma IL-18 level increased 1 h after hit with LPS, （ 6. 38 ± 1.62 ） pg/ml, it reached peak level at 4 h, （34.28 ± 17.89） pg/ml, and decreased rapidly at 8 h （ 14. 61 ±2.16） pg/ml. Lung tissue IL-18 level at 4 h increased significantly compared with that at 1 h, （508.96 ± 105. 38 ）pg/ml vs （346.08 ±21.23） pg/ml, P 〈0.01. Lung tissue IL-18 level was higher than plasma IL-18 level at the same time, P 〈0. 01. Conclusions IL-18 is an early pro-inflammatory cytokine and it may play an important role in the development of ALI.

关 键 词：内毒素 大鼠新生 IL-18 急性肺损伤 

分 类 号：R722.1[医药卫生—儿科]