兔肝辅酶Ⅱ依赖性视黄醇氧化还原酶全长cDNA的克隆与分析  

Cloning and Analysis of NADP(H)-dependent Retinol Dehydrogenase/Reductase Full-length cDNA in Rabbit Liver

作　　者：杜昆[1] 谢健平[1] 刘戈飞[1] 宋旭红[1] 黄东阳[1] 

机构地区：[1]汕头大学医学院分子生物学中心,广东汕头515041

出　　处：《汕头大学医学院学报》2006年第3期129-131,135,共4页Journal of Shantou University Medical College

摘　　要：目的:克隆兔肝辅酶Ⅱ依赖性视黄醇氧化还原酶(NRDR)的全长cDNA,并分析其特征。方法:根据GenBank中已发表的NRDR的cDNA部分序列信息,运用cDNA末端快速扩增技术得到全长cDNA,然后利用生物信息学的方法对其进行分析。结果:得到NRDR全长cDNA序列,并提交到GenBank。分析发现该cDNA具有短链脱氢酶(SDR)家族的保守模序,而且在5′端同一个读码框架内有2个起始密码子。结论:新克隆的NRDR全长序列在GenBank编号为DQ229110,NRDR蛋白属于SDR家族。Objective： To obtain and analysis the full-length cDNA of the NADP（H）-dependent retinol dehydrogenase/ reductase（NRDR）in rabbit liver. Methods： According to the partial sequences deposited in GenBank, the full-length cDNA was obtained using the techniques named rapid amplification of cDNA ends, and then, the sequence was analyzed bioinformatically. Results： The sequence was obtained and submitted to GenBank. The NRDR featured two conserved motifs of short chain dehydrogenase（SDR）family and possessed two initiation sites in the same reading frame. Conclusion： the accession number of NRDR Nil-length cDNA in GenBank is DQ229110, and the NRDR protein belongs to the SDR family.

关 键 词：视黄醇氧化还原酶 eDNA末端快速扩增 短链脱氢酶 维生素A代谢 

分 类 号：Q785[生物学—分子生物学]