高粱幼苗水分胁迫诱导表达差异cDNA的研究  

The Differential Expression of cDNA Induced by Water Stress in Sorghum Seedling

作　　者：张宏一[1] 朱志华[1] 景蕊莲[1] 

机构地区：[1]中国农业科学院作物科学研究所

出　　处：《植物遗传资源学报》2006年第3期277-283,共7页Journal of Plant Genetic Resources

摘　　要：以高粱为试验材料,用-0.7MPa的PEG-6000高渗溶液对其幼苗进行水分胁迫处理,利用mRNA差异显示技术分离得到53条高粱水分胁迫诱导表达的cDNA片段,其中包括5个完全诱导表达片段,43个上调片段和5个下调表达片段。经过Reverse Northern验证,筛选出13个差异表达的cDNA片段,并进行克隆测序。经GenBank查询,10个片段序列与已知序列有较高的同源性,3个片段同源性非常低,可能为新基因。mRNA differential display technique was used to isolate genes （cDNA） induced by -0.7MPa PEG-6000 in leaves of grain sorghum seedling. A total of 53 differential expression cDNA fragments had been detected,including 5 absolute expression fragments induced by water stress,43 enhanced expression fragments,5 inhibited expression fragments. After further verified with reverse northern dot blot, thirteen fragments are proved to be positive. Aliging with GenBank databases found that 10 cDNA fragments showed high homologous while other 3 were poor supposed new genes.

关 键 词：高粱 水分胁迫 基因表达 差异显示 

分 类 号：S514[农业科学—作物学]