小麦全蚀病菌拮抗细菌的筛选及鉴定  

Identification and Screening of Antagonistic Bacteria Against Take-all of Wheat

作　　者：罗兰[1] 袁忠林[1] 陈茎[1] 

机构地区：[1]莱阳农学院植物保护学院

出　　处：《莱阳农学院学报》2006年第3期205-207,共3页Journal of Laiyang Agricultural College

摘　　要：用对峙培养法从小麦根际中获得8株对小麦全蚀病有拮抗作用的菌株。这几株菌株对西瓜枯萎病、黄瓜枯萎病及棉花枯萎病也均具有拮抗作用。经培养性状、生理生化反应测定认为属于芽孢杆菌(Bacillus)。并对其中6株进行了分子鉴定,与枯草芽孢杆菌同源性达99%,可以认定是枯草芽孢杆菌(Bacillus subti-lis)。Eight antagonistic bacteria which showed suppress to take - all pathogen （ Gaeumannomyces graminis） were isolated form wheat rhizosphere soil. And all of these bacteria obviously inhibited 4 soil - borne disease pathogens （ Fusarium oxysporum f. sp. niveum,F, oxysporum f. sp. cucumerinum, F. oxysporum f. sp. vasinfectum） in laboratory. They were identified as Bacillus by its cultural morphological, physiological and biochemical characteristics. Six of these strains were identified as Bacillus subtilis based on 16SrDNA sequence, which shared 99% homologies with published sequence form GenBank.

关 键 词：小麦全蚀病 拮抗细菌 16srDNA 枯草芽孢杆菌 

分 类 号：S476.9[农业科学—农业昆虫与害虫防治]