PNA探针与DNA探针的系统比较  

A Systemic Comparison between PNA Probe and DNA Probe

作　　者：张兵波[1] 常津[1] 杨永[1] 张琦[1] 李双艳[1] 

机构地区：[1]天津大学材料学院纳米生物技术研究所,天津300072

出　　处：《高分子通报》2006年第9期62-68,共7页Polymer Bulletin

基　　金：国家自然科学基金(No:50373033);天津市应用基础研究重点基金(No:05YFJZJC01001)

摘　　要：肽核酸(Peptide Nucleic Acid,PNA)是近十几年发展起来的以中性酰胺键为骨架的脱氧核糖核酸(Deoxyribonucleic Acid,DNA)类似物,其结构介于多肽和DNA之间。由于PNA能够与DNA和RNA特异性地结合,可以制备PNA探针。与DNA探针相比,其杂交的稳定性和特异性增加且能在低盐浓度下进行杂交。本文从DNA和PNA的分子结构和性质、DNA探针和PNA探针的设计制备、杂交亲和性、杂交动力学以及在生物传感器上的应用等方面进行了系统比较。Peptide nucleic acid （PNA） with peptide linkages as its backbone is a brand new kind of compounds which are developed in recent years. It is an analog of DNA in molecular structure. PNA can hybridize DNA and RNA specifically, thus it can be used as probes. Compared with traditional DNA probes, PNA probes enjoy higher hybrid-stabilization, better specificity, and it can hybridize target substance under low salt ion concentration. This paper makes a systemic comparison between PNA probe and DNA probe in the aspects of probes preparation, hybridization specificity, hybridization kinetics and biosensors applications.

关 键 词：肽核酸 DNA 探针 杂交 系统比较 

分 类 号：Q78[生物学—分子生物学]