猪肝脏基因组DNA提取与纯化的研究  

Extraction and Purification of Genome DNA From Liver of Swine

作　　者：韩芬霞[1] 

机构地区：[1]河南科技学院动物科技学院,河南新乡453003

出　　处：《安徽农业科学》2006年第16期3980-3981,共2页Journal of Anhui Agricultural Sciences

摘　　要：对血液中提取基因组DNA的方法加以改进,获得了一种提取猪肝脏组织中DNA的有效方法。试验表明,利用SDS裂解,氯仿、异戊醇抽提母猪肝脏中基因组DNA,得到了纯度较高的DNA。A very simple and fast method for extracting high quality genomic DNA from liver of swine was described. The experiment indicated that DNA was extracted from swine by means of the application of the agents of SDS,chloroform and iso amyl alcohol so that a great deal of DNA required was acquired. The quantity and quality of the DNA was high enough for many biology experiments, such as PCR amplification and RAPD analysis.

关 键 词：母猪 肝脏 基因组DNA 提取 

分 类 号：S828[农业科学—畜牧学]