氧化苦参碱对肝纤维化大鼠肝组织细胞因子表达水平的影响  

Effect of oxymatrine on the hepatic expressions of cytokines in rats with hepatic fibrosis

作　　者：余小虎[1] 朱金水[2] 朱祖明[2] 朱励[2] 

机构地区：[1]上海市南汇区中心医院消化科,上海201300 [2]上海交通大学附属第六人民医院消化科

出　　处：《实用肝脏病杂志》2006年第5期257-259,共3页Journal of Practical Hepatology

摘　　要：目的研究氧化苦参碱治疗大鼠肝纤维化过程中肝组织细胞因子IL-2、IL-10、IFN-γ表达水平的变化。方法采用皮下注射四氯化碳及饲以10%酒精的方法,使Wistar大鼠产生肝纤维化,纤维化大鼠随机分成二组,分别为自然恢复组(R组)和氧化苦参碱治疗组(T组),免疫组化法检测肝组织IL-2、IL-10、IFN-γ的水平。结果肝纤维化大鼠肝组织IL-10含量明显增高,而IL-2与IFN-γ则明显下降;氧化苦参碱治疗9周后,在肝纤维化程度明显改善的同时,上述细胞因子的异常亦明显纠正。结论氧化苦参碱逆转肝纤维化作用可能与其免疫调节功能有关。Objective To study the changes of hepatic expressions of IL-2, IL-10, IFN-γ after the treatment of oxymatrine on hepatic fibrosis in rats. Methods Hepatic fibrosis was developed in Wistar rats within 8 weeks using subcutaneous injection of carbon tetrachloride（twice a week . 3ml · k^-1·g^-1）and feeding with alcohol （containing 10% ethanol）, then the rats with hepatic fibrosis was randomly divided into two groups treated with peritoneal injection of oxymatrine（100mg · k^-1·g^-1,T group ） and normal saline（R group） respectively for consecutive 9 weeks. The levels of IL-2, IL-10, IFN-γ in liver tissues were measured using immunohistochemistry assays and image analysis system at the end point. Results The level of IL-10 in rats with hepatic fibrosis were markedly higher than that in normal rats, whereas the levels of IL-2 and IL-10 were markedly decreased after the therapy of oxymatrine, with the severity of hepatic fibrosis reduced. The abnormity of all the above cytokines were markedly improved too. Conclusion The therapeutic effects of oxymatrine on hepatic fibrosis may be correlated to its immunological regulation.

关 键 词：肝纤维化 氧化苦参碱 白介素2 白介素10 γ干扰素 大鼠 

分 类 号：R285.5[医药卫生—中药学]