体外培养的人正常子宫内膜细胞中PTEN和Bim蛋白的表达  

Expressions of PTEN and Bim in human normal endometrial cells cultured in vitro

作　　者：杨清[1] 王光伟[1] 王玉[1] 

机构地区：[1]中国医科大学附属第二医院妇产科,辽宁沈阳110004

出　　处：《中国医科大学学报》2006年第4期362-363,F0002,共3页Journal of China Medical University

基　　金：辽宁省自然科学基金资助项目(20032064)

摘　　要：目的:探讨PTEN和B im蛋白在体外培养的人正常子宫内膜细胞中的表达情况。方法:选取正常的新鲜子宫内膜组织10例,通过胰蛋白酶消化,培养原代及传代子宫内膜细胞,并利用免疫细胞化学染色定性,定位及半定量检查细胞受体、PTEN和B im的表达。结果:体外培养的子宫内膜细胞中,雌、孕激素受体、PTEN、B im均表达阳性。结论:体外培养的人正常子宫内膜细胞保持了正常的生物学特性,重要的促凋亡蛋白PTEN、B im及雌、孕激素受体并未丢失。Objective： To investigate the expressions of PTEN and Bim in human normal endometrial cells cultured in vitro. Methods： Fresh endometrial specimens which were histologically normal were collected from 10 patients with uterine myoma and digested by trypsin. The primary endometrial cells were cultured and passaged. The expressions of cell surface receptor, PTEN, and Bim were determined by qualitative, site-specific, and simiquantitative immunocytochemistry. Results： The expressions of estrogen and progesterone receptors, PTEN, and Bim were found in all the endometrial cells. Conclusion： Endometrial cells cultured in vitro maintain normal biological characteristics, and PTEN, Bim, and estrogen and progesterone receptors are not lost.

关 键 词：子宫内膜 细胞培养 PTEN BIM 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]