龙胆泻肝汤对四氯化碳肝损伤大鼠肝脏转运功能的影响  

Influence of Longdanxiegan on the Hepatobiliary Transportation in Rats treated byCarbon Tetrachloride

作　　者：石红[1] 周琰[1] 何士彦[1] 张凤华[2] 张建平[3] 李军云[3] 武梅芳[3] 徐华洲[3] 李乾[3] 

机构地区：[1]石家庄市中心医院,河北石家庄050011 [2]河北医科大学中西医结合学院,河北石家庄050091 [3]河北医科大学基础课教学部,河北石家庄050091

出　　处：《辽宁中医杂志》2006年第8期1041-1042,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：河北省科技厅科研课题(06276166)

摘　　要：目的:研究龙胆泻肝汤对四氯化碳(CC l4)所致急性肝损伤大鼠的靛氰绿(ICG)肝清除率的影响。方法:将32只大鼠随机分为空白对照组、模型组和龙胆泻肝汤高、低剂量组,每组8只。用CC l4制作大鼠急性肝损伤模型,测定下列变化:(1)大鼠血清天冬氨酸转氨酶(ALT)、丙氨酸转氨酶(AST);(2)肝脏形态学的改变;(3)肝血流量和靛氰绿肝清除率的改变;观察龙胆泻肝汤对CC l4大鼠急性肝损伤的影响。结果:龙胆泻肝汤能明显抑制CC l4所致大鼠血清中ALT及AST含量的升高(P<0.05或P<0.01),改善肝脏组织病理,对抗CC l4所致肝血流量和肝清除率下降(P<0.05)。结论:龙胆泻肝汤可保护肝脏,对抗CC l4所致肝清除率和肝血流量下降。Objective ： To study the influence of Longdanxiegan on the hepatobiliary transportation in rats treated by carbon tetrachloride （ CC14 ）. Methods ： 32 rats were randomly allocated to normal control group, model group and Longdanxiegan groups （low - and high - dosage ）. The following studies were carried out： （ 1 ） Determination of the serum levels of aspartate transaminase （AST） and alanine transaminase （ ALT ） ; （ 2 ） Observation of the patho physiological changes and （3） Determination of hepatic plasma flow and ICG hepatic clearance by a continuous indocyanine green （ICG） infusion method. Results ：Longdanxiegan significantly reduced acute liver injury induced by CC14, decreased ALT and AST in serum （ P 〈 0.05 or P 〈 0.01 ） , improved the pathophysiologica]. changes, antagonised the decreses in the hepatic plasma flow and hepatic clearance of ICG in CC14 - treated rats （ P 〈 0.05 or P 〈 0.01 ）. Conclusion ： Longdanxiegan has protecting effects for the acute hepatic injury induced by CC14, antagonise CCla - induced decreses in the hepatic plasma flow and hepatic clearance of ICG.

关 键 词：龙胆泻肝汤 四氯化碳 靛氰绿 肝清除率 

分 类 号：R285.5[医药卫生—中药学]