苯甲曲秦片剂含量及溶出度反相HPLC测定  

Determination and dissolution of phendimetrazine tablet by RT-HPLC

作　　者：刘辽[1] 杨云霞[1] 彭波[2] 包定元[1] 

机构地区：[1]四川大学基础与法医学院药理教研室,四川成都610041 [2]中山大学生命科学院动物生理教研室,广东广州510275

出　　处：《西部医学》2006年第5期583-585,共3页Medical Journal of West China

摘　　要：目的 建立苯甲曲秦片剂的含量、溶出度反相HPLC测定方法。方法Capcell-pak C18色谱柱，甲醇／1-庚烷磺酸钠缓冲液为流动相，检测波长为256nm，流速1．0ml／min，以水杨酰胺作为内标计算含量。Capcell-pak C18色谱柱，乙晴/0.025mol／L磷酸二氢钾溶液缓冲液为流动相，检测波长为210nm，流速1.0ml／min，外标法计算溶出度。结果 含量试验线性范围为：9.375～150mg／100ml，r=0.9999，回收率为103．35％，RSD为0．61％。溶出度线性范围为：1．25～20mg／100ml，r=0．9999。结论 该条件下反相HPLC测定苯甲曲秦片剂的含量、溶出度方法快速简便。Objective To establish a method for determination and dissolution of phendimetrazine tablets by RT-HPLC. Methods Using RT-HPLC with Capcell-pak C18 column,methanol/sodium 1-heptane sulfonate buffer,as the mobile phase. Detection wavelength was 256 nm. Results Calibration curves were linear in the range of 9. 375-150 mg/100ml^-1 for determination and 1.25-20 mg/100ml^-1 for dissolution test（r=0. 9999）. Conclusion The method is simple, rapid and the results are accurate,reliable.

关 键 词：苯甲曲秦 HPLC 含量 溶出度 

分 类 号：R927.2[医药卫生—药学]