定量RT-PCR分析穿孔素基因在无症状乙型肝炎病毒携带者中的表达及意义  

Analysis of Expression of Perforin in Asymptomatic HBV Carriers of by Quantitative RT-PCR

作　　者：唐翠兰[1] 周舟[2] 陈智[1] 

机构地区：[1]浙江大学第一附属医院传染病研究所,浙江杭州310031 [2]新乡医学院第三附属医院,河南新乡453003

出　　处：《中国误诊学杂志》2006年第17期3280-3282,共3页Chinese Journal of Misdiagnostics

基　　金：本课题获浙江省科技厅重大项目(编号:2003C13015)

摘　　要：目的:验证无症状乙型肝炎病毒携带者(A SC s)与正常对照组消减文库中的差异基因穿孔素(perforin)在相应人群中的差异表达。方法:采集43例A SC s和41例正常对照者的外周静脉血,分离外周血单个核细胞(PBM C)并提取RNA,做定量RT-PCR(R ea l-tim e RT-PCR)。结果:穿孔素在HBV携带者中表达水平是正常对照的3.58倍。结论:通过定量RT-PCR首次证实了穿孔素基因在A SC s较正常对照表达增高,因穿孔素与免疫抑制有关,该基因在A SC s的高表达对于阐明A SC s的发病机制有重要意义。Objective：To identify the differentially expression of the gene perforin from the subtracted libraries between asymptomatic HBV carriers （ASCs） and the normal control subjects. Methods：2- 5 ml venous blood was collected from the subjects of 43 ASCs and 41 the normal control, peripheral blood mononuclear cells （PBMC） were isolated freshly,RNA was extracted from the PBMC for real time RT-PCR. Results：Perforin was confirmed as up expressed by 3.58 fold in ASCs subjects compared to the normal control subjects by real time RT-PCR. Conclusion：Perforin is first confirmed as over-expressed in ASCs compared to the normal control,for perforin is associated with depression of immunity, so the over-expression of perforin in ASCs can play important role in the mechanisms of ASCs

关 键 词：肝炎病毒 乙型 带病原状态 膜糖蛋白类/遗传学 逆转录聚合酶链反应 

分 类 号：R512.6[医药卫生—内科学]