日本血吸虫卵壳蛋白基因的克隆及真核表达载体的构建  

CLONING AND CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR OF EGGSHELL PROTEIN GENE FROM SCHISTOSOMA JAPONICUM CHINESE STRAIN

作　　者：张慧[1] 苑纯秀[1] 冯新港[1] 蔡幼民[1] 林矫矫[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所

出　　处：《寄生虫与医学昆虫学报》2006年第3期135-138,共4页Acta Parasitologica et Medica Entomologica Sinica

基　　金：国家"863"计划资助项目(No.2004AA2Z3510);科技部科研院所社会公益研究专项(No.2004DIB4J158);上海市重大科技攻关项目(No.03DZ19231)

摘　　要：血吸虫卵壳蛋白基因是探讨血吸虫病免疫预防的重要靶标。本研究根据已发表的多个血吸虫卵壳蛋白基因的核苷酸序列,设计合成了特异引物,体外扩增和克隆了编码日本血吸虫中国大陆株卵壳蛋白基因SjESG的cDNA,测序结果表明与已发表的卵壳蛋白基因序列有较高同源性。为进一步研究该基因的功能,将其克隆入pcDNA3中,成功构建了真核表达载体,为SjESGDNA疫苗的研究打下了基础。The egg plays a central role in the biology of Schistosoma japonicum and is responsible for dissemination of the parasite. Therefore, the egg and the female reproductive system are potential targets for pharmacological attack and/or vaccine development. Aimed at exploring the egg related gene and its function, and understanding the molecular mechanisms of egg formation, development and mature process and developing new candidate S. japonicum vaccine antigens, one gene encoding eggshell protein SjESG was amplified and obtained amplified fragment with 639 base pairs. And then the cloning plasmid （pSK （t） -SjESG） and expression plasmid （pcDNA-SjESG） contained the amplified fragment were also constructed. Results demonstrated that the amplified fragment was identical with expected one, and the eukaryotic expression plasmid was successfully constructed and might be useful for further study on DNA vaccine of S. japonicum.

关 键 词：日本血吸虫 卵壳蛋白 克隆 真核表达载体 

分 类 号：Q958.9[生物学—动物学] Q78