高效液相色谱法测定三黄片中盐酸小檗碱的含量  

Determination of Berberine Hydrochloride in Sanhuang Tablets by HPLC

作　　者：黄海燕[1] 薛漓[1] 

机构地区：[1]广西壮族自治区桂林食品药品检验所,广西桂林541002

出　　处：《中国药业》2006年第10期18-19,共2页China Pharmaceuticals

摘　　要：目的建立三黄片中盐酸小檗碱的含量测定方法。方法高效液相色谱法,色谱柱为KromasilTMC18柱,每1000mL含磷酸二氢钾3.4g、十二烷基硫酸钠1.7g的乙腈-水(65∶35)为流动相,流速1mL/min,检测波长345nm。结果盐酸小檗碱进样量线性范围是0.0357～0.357μg,r=0.9999,平均回收率为100.3%,RSD=0.77%(n=6)。结论该法操作简便、快捷、准确,可用于三黄片的质量控制。Objective To establish the assay of berberine hydrochloride in Sanhuang Tablets（Radix et Rhizoma Rhei, Extractum scurellariae and berberine hydrochloride）.Methods HPLC was used. Chromatographic conditions included KromasilTM C18（250 mm×4.6 mm, 5μm） column. The mobile phase was acetonitrile-water（65 ： 35）, containing potassium dihydrogen phosphate 3.4 g and sodium dodecyl sulfate 1.7 g per 1 000 mL. The UV detection wavelength was at 345 nm. Results The calibration curves was linear in the range of 0.035 7-0. 357μg for berberine hydrochloride（r=0. 9999）. The average recovery rate was 100.3% （RSD=O. 77%, n=6）. Conclusion The method is convenient, fast and accurative.

关 键 词：三黄片 盐酸小檗碱 高效液相色谱法 含量 

分 类 号：R286.0[医药卫生—中药学] R284.1[医药卫生—中医学]