小鼠腹水瘤巨噬细胞对大肠杆菌O157免疫应答的膜蛋白质组学研究  

Applying Subproteomics Methodies to Study the Immunoresponse of Macrophage Raw 264.7 Cells to Nosogenetic EHEC O157:H7-EDL933 Bacterium

作　　者：张国林[1] 杨天赐[1] 黄建伟 王三英[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]厦门市疾病控制中心,福建厦门361004

出　　处：《厦门大学学报（自然科学版）》2006年第5期596-601,共6页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30080029)资助

摘　　要：采用膜亚蛋白质组学方法研究小鼠腹水瘤巨噬细胞Raw 264.7对致病性大肠杆菌O157的免疫应答反应,筛选并鉴定到24个差异表达的膜蛋白质.这些膜蛋白质与细胞免疫、细胞周期、细胞发育、细胞骨架、细胞生长等有关.在蛋白质水平上对巨噬细胞与大肠杆菌间的相互作用关系进行了一些探索性研究,为进一步揭示机体对病原反应的作用机制提供理论基础.Subproteomics approach is applied to investigate the change of membranous proteins expression of Raw 264.7 macrophage immunoresponse to the nosogenetic EHEC O157：H7-EDL933 bacterium. Cells death and growth are observed after incubating with E. coli for 6 h in this study,and 24 differential expression membranous proteins are idendified with proteomics approach,which involved in cellular immunity, cell cycle, cellular development, cytoskeleton and cell growth. Seven are involved in interaction between cells and bacterium, the other twelve are involved in various process,which strongly suggests the molecular mechanism of immunoresponse between cell and bacterium. Our results on interaction between cells and bacterium provide the theoretical evidence for elucidating the mechanism of response of organism to pathogen.

关 键 词：细胞微生物学 蛋白质组学 巨噬细胞 大肠杆菌 

分 类 号：Q939.91[生物学—微生物学]