脑热清对内毒素性发热家兔解热作用的实验研究  

Effect of Naoreqing on endotoxin-induced fever in rabbits

作　　者：刘智勤[1] 蒋玉凤[2] 岳晓莉[2] 樊永平[3] 王玺玫[2] 黄启福[2] 

机构地区：[1]河北大学药学院,河北保定071002 [2]北京中医药大学病理教研室,北京100029 [3]中国医学科学院北京天坛医院,北京100050

出　　处：《中国病理生理杂志》2006年第9期1816-1819,共4页Chinese Journal of Pathophysiology

基　　金：北京市科委课题资助(No. 954024200)

摘　　要：目的:探讨脑热清(NRQ)口服液对内毒素性发热家兔的解热作用。方法:复制大肠杆菌内毒素性发热家兔模型,用数字温度计测量家兔的直肠温度,并采用放免法测定血浆内皮素的含量及血清肿瘤坏死因子(TNFα)的含量,用发色底物法测定血浆组织纤溶酶原激活物(t-PA)和组织纤溶酶原激活物抑制剂(PAI)的活性,同时取肺、肾、肝组织,观察形态改变。结果:①NRQ对内毒素性发热家兔有显著的解热效应(P<0.01);②NRQ可明显抑制发热家兔内皮素、TNFα的含量上升(P<0.01),同时可降低PAI活性(P<0.01)、提高t-PA的活性(P<0.01);③光镜显示,NRQ可减轻发热家兔肺、肾、肝血管扩张充血、淤血病理形态的改变。结论:NRQ可能通过调节发热家兔促凝和抗凝物质的平衡、抑制致热性细胞因子释放达到解热作用。AIM ： To observe the antipyretic effect of Naoreqing （NRQ） oral solution in rabbits with endotoxin - induced fever. METHODS： The ET- induced fever model was established in rabbits. The digital thermometer was used to register rectal temperature. The endothelin content in blood plasma, and tumor necrosis factor - ot （TNFot） in blood serum were determined by radioimmunoassay. The activities of tissue - type plasminogen activator （ t - PA） and plasminogen activator inhibitor （PAI） in plasma were measured by the assay of chromogenic substrate. At the same time, the pathology of lung, kidney and liver were observed. RESULTS： ① NRQ showed obvious antipyretic effect in the rabbits with endotoxininduced fever （P 〈0. 01 ）. ② NRQ inhibited the endothelin content in plasma and TNFα content in serum, and at the same time reduced activity of PAI （ P 〈0. 01 ） and improved activity of t - PA in plasma （ P 〈0. 01 ）. ③ NRQ reduced the pathology of congestion of lungs, liver and renal in febrile rabbits. CONCLUSION： NRQ has an antipyretic effect . The mechanism is maybe related to its adjusting the balance of coagulant substance and anticoagulant substance, and at the same time inhibiting the secretion of TNFα in febrilic rabbits.

关 键 词：内毒素类 发热 家兔 中草药 

分 类 号：R285.5[医药卫生—中药学]