HSP70对大鼠肝脏局部缺血再灌注后炎症反应的影响  

Effects of heat shock protein 70 on inflammation after hepatic local ischemia/reperfusion in rats

作　　者：安冬艳[1] 赵祯[1] 曹宇[1] 

机构地区：[1]中国医科大学生理教研室,辽宁沈阳110001

出　　处：《中国病理生理杂志》2006年第9期1820-1824,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30270198);沈阳市自然科学基金资助项目(No.041129)

摘　　要：目的:通过热应激预处理诱导HSP70表达,探讨其对肝脏缺血再灌注损伤后炎症反应的影响。方法:采用大鼠局部缺血再灌注模型(IR组),并在热应激预处理(H+IR组)及槲皮素+热应激预处理(Q+H+IR组)条件下观察肝脏缺血再灌注后HSP70、ICAM-1的表达及MPO的活性;测定血清ALT和AST的活性;电镜观察肝细胞结构的改变。结果:在H+IR组检测的各时点HSP70表达均明显高于其它两组、对肝脏进行缺血再灌注后,肝细胞损伤较轻,血清ALT、AST升高不明显(P<0.01);肝组织中ICAM-1表达增加,以再灌注后6h最显著,MPO活性升高以12h最为显著,但两者变化均低于IR组和Q+H+IR组(P<0.01)。结论:热应激预处理诱导产生HSP70蛋白能够降低大鼠肝脏缺血再灌注损伤过程ICAM-1的表达和MPO活性的改变,进而抑制炎症反应引起的肝脏损伤。AIM ： To study the effects of heat shock protein 70 （ HSP70 ） induced by the heat stress pretreatment on inflammation after hepatic ischemia/resperfusion. METHODS： With the hepatic local ischemia/reperfusion model （IR group）, heat stress pretreatment （ H ＋ IR group） and injecting quercetin before heat stress pretreatment （ Q ＋ H ＋ IR group） were performed. The HSP70, intercellular adhesion molecule - 1 （ICAM - 1 ） and the myeloperoxidase （MPO） activity were detected. The levels of serum ALT and AST and histological changes of the hepatocytes were also observed. RESULTS： In H ＋ IR group, the HSP70 expression was higher than that in other groups at each time point, after performing ischemia - perfusion, hepatic injury was slighter. The levels of serum ALT and AST were increased slightly （ P 〈 0. 01 ）. The expression of ICAM - 1 and the changes of MPO activity increased and peaked respectively at 6 h and 12 h after reperfusion. However, they were lower in H ＋ IR group than those in IR group and Q ＋ H ＋ IR group. CONCLUSION： The HSP70 induced by heat stress pretreament reduces the expression of ICAM - 1 and the changes of MPO activity during hepatic ischemia - reperfusion, then hepatic injury is depressed from the inflammation.

关 键 词：热休克蛋白质70 再灌注损伤 胞间粘附分子1 过氧化物酶 肝 

分 类 号：R657.3[医药卫生—外科学]