IFN-α对宫颈癌细胞MHC-I类链相关蛋白A表达的上调作用  

Up-regulation of IFN-α on expression of MICA in the cervical carcinoma cell lines

作　　者：牛家峰[1] 张彩[1] 张建华[1] 许晓群[1] 张维东[1] 王郡甫[1] 高春义[1] 

机构地区：[1]山东省医学科学院基础医学研究所

出　　处：《细胞与分子免疫学杂志》2006年第5期557-559,563,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30371302);山东省自然科学基金资助项目(Y2002C24);山东省优秀中青年科学家科研奖励基金项目(2005BS03015)

摘　　要：目的:观察IFNα对宫颈癌细胞MHCI类链相关蛋白A(MICA)表达及功能的影响。方法:以IFNα诱导宫颈癌细胞系HeLa和Caski后,用半定量RTPCR和免疫组化染色法观察诱导前后MICAmRNA和其蛋白表达的变化,用MTT比色法检测诱导前后宫颈癌细胞对外周血NK细胞杀伤敏感性的变化。结果:经IFNα诱导后,HeLa和Caski细胞中MICAmRNA和其表面MICA蛋白的表达水平均明显上调,并呈一定的时间和剂量依赖关系。NK细胞对MICA表达较高的HeLa细胞的杀伤活性,明显高于对MICA弱表达的Caski细胞。经1×106U/LIFNα诱导3d后,两种细胞对NK细胞杀伤的敏感性均明显增强(P<0.01)。结论:IFNα可上调MICA在肿瘤细胞表面的表达,并可增强肿瘤细胞对NK细胞杀伤的敏感性。AIM： To investigate the effect of IFN-α on expression of MHC class I chain-related protein A （MICA） in the cervical carcinoma cell lines, METHODS： The cervical carcinoma cell lines （HeLa and Caski） were treated with IFN-α. The expression of MICA was measured by RT-PCR and by immunohistochemical staining. The cytotoxicity of human NK cells to the IFN-α treated cervical carcinoma cells was detected by M＇IT method. RESULTS： The mRNA and protein expression of MICA was up-regulated by IFN-α in HeLa and Caski cells in a time and dose-dependent manner. Compared to Caski cells which weakly experessed MI- CA, higher cytolytic activity of NK cells was found against HeLa cells, which experessed relatively higher level of MICA. After being treated with IFN-α for 3 d, the susceptibility of the two cervical carcinoma cells to NK cytolysis was increased significantly. CONCLUSION： IFN-α can up-regulate the MICA expression in the cervical carcinoma cell lines and thereby enhance the susceptibility to cytolysis of NK cells.

关 键 词：宫颈癌 IFN-Α MICA NK细胞 NKG2D 

分 类 号：R392.12[医药卫生—免疫学]