槲皮素抑制人胃癌MGC-803细胞增殖并诱导其凋亡的研究  

Quercetin inhibits growth and induces apoptosis of human gastric carcinoma cells

作　　者：王海燕[1] 郭良淼[1] 陈勇[1] 赵雪花[1] 成彩莲[1] 吴勉云[1] 何丽娅[1] 

机构地区：[1]武汉科技大学医学院细胞与分子生物学研究所,湖北武汉430080

出　　处：《细胞与分子免疫学杂志》2006年第5期585-587,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：湖北省教育厅科研重点资助项目(D200511007)

摘　　要：目的:研究槲皮素抑制人胃癌MGC803细胞增殖和诱导凋亡的作用。方法:采用MTT比色法检测不同终浓度的槲皮素对MGC803细胞增殖的影响及其细胞毒活性。应用TUNEL(TdTmediateddUTPnickendlabeling)染色法检测槲皮素诱导MGC803细胞凋亡的作用。用免疫组化法测定P16、P53和Cmyc的表达率。结果:在40～100μmol/L范围内,槲皮素可显著抑制MGC803细胞增殖(P<0.01),且呈剂量、时间依赖性。TUNEL染色显示,槲皮素诱导48h后,MGC803细胞的凋亡率明显高于对照组(P<0.01)。免疫组化检测表明,用药组P53及Cmyc蛋白的表达下降,P16蛋白的表达率增强,与对照组相比较均有显著性差异(P<0.01)。结论:槲皮素可抑制人胃癌MGC803细胞增殖,并能诱导其凋亡,其作用机制可能与下调P53及Cmyc蛋白的表达、上调P16蛋白的表达有关。AIM： To study the effect of quercetin on the growth and apoptosis of human gastric carcinoma cell line MGC-803. METHODS： The measurement of inhibitory rate and apoptotic index（AI） of quercetin were done by MTT assay and TUNEL assay. The positive expression rate of P53, C-myc and P16 were detected by immunocytochemical staining. RESULTS： Quercetin at concentrations ranging from 40μmol/L to 100μmol/L significantly inhibited the proliferation of MGC-803 cells in a dose- and time-dependent manner （P〈0.01）. TUNEL assay indicated that the number of apoptotic cells in quercetin-treated group was greater than that in the control group （P〈0.01）. Expression of P53 and C-myc protein decreased following quercetin induction in a dose-dependent manner, whereas P16 expression increased significantly compared with that of the control group （ P〈0.01 ）. CONCLUSION： Quercetin can inhibit the growth and induce apoptosis of MGC-803 cells in a dose- and timedependent manner. Its mechanisms may be relevant to the down-regulation of P53 and C-myc protein expression as well as up-regulation of P16 expression.

关 键 词：槲皮素 MGC-803细胞 抑制作用 凋亡 蛋白表达 

分 类 号：R33[医药卫生—人体生理学]