Hsp701A基因的RNA干涉对HepG2细胞化疗敏感性的影响  

Increased sensitivity to chemotherapeutic drug by RNAi targeting Hsp701A in HepG2 cells

作　　者：朱青[1] 张王刚[2] 王立锋[3] 王芳[3] 张勇[3] 杨安钢[3] 

机构地区：[1]西安交通大学医学院第一附属医院肿瘤中心,陕西西安710061 [2]西安交通大学医学院第二附属医院血液内科,陕西西安710004 [3]第四军医大学免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2006年第5期606-608,612,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金项目(30200369);陕西省科学技术发展计划项目(2003K10G39)

摘　　要：目的:研究Hsp701A基因靶向siRNA表达载体对肿瘤细胞化疗药物敏感性的影响。方法:构建Hsp701A靶向小干涉RNA(siRNA)的真核表达载体,并转染HepG2细胞,以证实siRNA真核表达载体RNAi的有效性。用四唑蓝(MTT)比色法检测HepG2细胞对白藜芦醇、顺铂的敏感性。结果:以构建的Hsp701A基因靶向siRNA的真核表达载体稳定转染HepG2细胞后,两种RNAi组细胞中Hsp701ARNA和蛋白的表达水平均明显下降。Hsp701A基因靶向siRNA真核表达载体转染后,HepG2细胞对不同化疗药物的敏感性有不同程度的增加(P<0.05)。结论:Hsp701A基因的RNA干涉可增强HepG2细胞对化疗药物的敏感性。AIM： To investigate the effects on chemotherapeutic sensitivity to the drugs of tumor cells induced by Hsp701A targeting siRNA expression vector. METHODS：Specific small interference RNA （siRNA） targeting Hsp701A gene was generated and its eukaryotic expression vector was constructed and transfected into human HepG2 cells. Sensitivity to the chemotherapeutic drug of HepG2 cells transfected with the vector was detected by MTT colorimetry. RESULTS： The eukaryotic expression vector of siRNA targeting Hsp701A was successfully constructed and transfected into human HepG2 cells, Sensitivity to the chemotherapeutic drug of HepG2 cells was increased after Hsp701A was knock down （ P 〈 0. 05 ）. CONCLUSION： Knockdown of Hsp701A gene by siRNA enhances sensitivity to chemotherapeutic drug in HepG2 cells.

关 键 词：热休克蛋白701A RNA干涉 HEPG2细胞 药物敏感性 

分 类 号：Q342.3[生物学—遗传学]