家族聚集性慢性乙型病毒性肝炎患者PBMC免疫相关的基因表达谱  

Expression map of immune-related genes on PBMCs in familial clustering patients with chronic virus hepatitis B

作　　者：何英利[1] 赵英仁[1] 张树林[1] 杨瑗[1] 刘敏[1] 金燕[1] 刘锦锋[1] 

机构地区：[1]西安交通大学第一医院感染科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2006年第5期620-622,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(30270597)

摘　　要：目的:应用寡核苷酸基因芯片技术,研究家族聚集性HBV感染者外周血单个核细胞(PBMC)免疫相关基因的表达谱。方法:在一聚集性HBV感染家族中,选取患者5例和患者的正常配偶4例,提取PBMC中的RNA,与涵盖2.2万个EST的寡核苷酸表达谱基因芯片U133A2.0杂交,通过Affymetrix扫描仪和DNT分析软件比较患病组与对照组PBMC免疫相关基因的的表达谱,获得基因的相对表达比值。结果:在2.2万个EST中,初筛出24个免疫相关差异表达基因,7个基因表达上调,17个表达下调。上调的主要是与适应性免疫相关的基因,下调的主要是与固有免疫相关的基因。结论:宿主感染HBV后,可改变其免疫基因表达,固有免疫功能的障碍可能是HBV感染慢性化的主要原因。AIM： To investigate peripheral blood mononuclear cells （PBMCs） immune-related gene expression profile in patients with chronic virus hepatitis B（CHB） by oligonucleotide gene array technique. METHODS： PBMCs were collected from the members of a family of clustering hepatitis B virus （HBV） infection including 5 CHB patients and 4 healthy spouses and RNA prepared from PBMCs was hybridized to high-density oligonucleotide arrays（ HG-U133A 2.0 Human Gene Chips, Affymetrix）, covering the expres- sion of 22 000 human ESTs. analyzed with DNT software the 22 000 ESTs, 24 different Primary scanned image was package. RESULTS： Out of immune-related genes were identified. Among the 24 genes, 7 genes showed increased expression and 17 genes showed decreased expression in CHB compared with those in healthy spouses. The up-regulated genes were mainly associated with adaptive immunity, while the down-regulated genes were associated with innate immunity. CONCLUSION： Our findings suggest that HBV infection alters a broad range of immunity genes expression and innate immunity-associated genes are important in the defense against HBV chronic infection.

关 键 词：寡核苷酸基因芯片 乙型肝炎病毒 先天性免疫 适应性免疫 

分 类 号：R512.62[医药卫生—内科学]