自身免疫甲状腺病患者血清中IL-12和IL-18水平的分析  

Measurement of IL-12 and IL-18 in sera of patients with autoimmune thyroid disease

作　　者：张进安[1] 张健[2] 徐利[1] 买尔哈巴[1] 吴哓燕[1] 

机构地区：[1]西安交通大学医学院第一附属医院内分泌科,陕西西安710061 [2]西安交通大学医学院第一附属医院检验科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2006年第5期630-632,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省科技攻关资助项目(2002K10G5)

摘　　要：目的:提供自身免疫甲状腺病(AITD)患者体内Th1/Th2平衡紊乱的依据。方法:应用酶联免疫吸附法(ELISA)测定27例Graves病(GD)、24例甲状腺功能正常的桥本甲状腺炎(HT)、25例甲状腺功能低下的HT患者及20例正常对照者血清中IL12和IL18的浓度,并检测GD患者的甲状腺刺激性抗体。结果:GD患者与甲状腺功能正常的HT患者血中IL12、IL18水平无明显差异,但均高于正常对照者的相应水平。甲状腺功能低下的HT患者血中IL12和IL18的水平与正常对照者无差异。在GD和甲功正常的HT,IL18与IL12呈明显正相关。在GD,IL12和IL18均与其甲状腺刺激性抗体(TSAb)活性呈正相关。在甲状腺功能正常的HT还存在IL12和IL18二者与甲状腺球蛋白抗体(TgAb)的显著性正相关。结论:提示Th1型细胞在GD和HT两种AITD的发病中均起重要作用。通过抑制Th2型免疫反应,促进向Th1型的转变来治疗GD时,有可能导致病情恶化。AIM： To provide some evidence for the imbalance of Th1/Th2 cells in patients with autoimmune thyroid disease （AITD）, METHODS： Concentrations of IL-12 and IL-18 in the serum samples of 27 patients with Graves＇ disease（ GD）, 24 patients with euthyroid Hashimoto＇s thyroiditis （HT）, 25 patients with hypothyroid HT and 20 normal controls were investigated by ELISA. The activity of thyroidstimulating antibody （TSAb） was also assayed in GD patients. RESULTS： The mean levels of IL-12 and IL-18 in patients with GD and euthyroid HT were significantly higher than those in normal controls. Significant positive correlations were found between the level of IL-12 and IL-18 in the two groups. In addition, striking positive correlations were noted between the levels of IL-12, IL-18 and the activity of TSAb in GD, as well as between the levels of IL-12, IL-18 and the titers of anti-thyroglobulin antibody（TgAb） in euthyroid HT. However, no differences were observed in IL-12 and IL-18 levels between hypothyroid HT and normal controis. CONCLUSION： Thl-type cell and related cytokines might play an important role in the pathogenesis of HT and GD, Such therapies can aggravate the process of disease in GD by suppressing Th2 response and modulating imbalance toward Th1 predominance.

关 键 词：Graves病 桥本甲状腺炎 IL-12 IL-18 TH1/TH2 

分 类 号：R581[医药卫生—内分泌]