兔抗Nogo-66受体(NgR)LRR片段抗体的制备和鉴定  

Preparation and identification of rabbit antibody against LRR of Nogo-66 receptor (NgR)

作　　者：于盼盼[1] 朱慧琴[1] 陆佩华[1] 黄立东[1] 

机构地区：[1]上海交通大学医学院神经生物学实验室,上海200025

出　　处：《细胞与分子免疫学杂志》2006年第5期633-635,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：上海市科技发展基金资助项目(02JC14014)

摘　　要：目的:制备兔抗Nogo66受体(NgR)片段LRR的抗体,并进行特性鉴定。方法:采用原核系统表达大鼠NgR的LRR片段,经纯化后免疫新西兰兔制备抗LRR的抗体,分别用Westernblot、免疫组化染色法检测抗体的效价和特异性。结果:获得高效价的兔抗大鼠LRR的抗体,该抗体能够识别大鼠脑和脊髓组织中的NgR分子。脊髓切片的免疫组化染色结果显示,NgR分子在神经元中广泛表达。结论:制备出能特异性识别天然NgR分子的抗体,为检测NgR分子并进一步研究其功能奠定了基础。AIM： To prepare rabbit antibody against LRR of Nogo-66 receptor （NgR） and identify its properties. METHODS： LRR protein was expressed in E. coli BL21. Rabbits were immunized with purified LRR protein to prepare anti-LRR antibody, The titer and specificity of prepared antibodies were identified by Western blot and immunohistochemical staining, respectively, RESULTS： Purified rabbit anti-rat LRR antibody with high titer was obtained. The anti- LRR Ab showed good specificity and could be used to detect the NgR in rat brain and spinal cord tissues. Immunohistochemical staining indicated that NgR was expressed widely in spinal cord neurons. CONCLUSION： Successful preparation of anti-rat LRR antibody provides a useful tool for identification and further functional study of NgR molecule.

关 键 词：神经再生 NGR 抗体 兔 

分 类 号：R392.11[医药卫生—免疫学]