抗人DR5单克隆抗体对人肝细胞系HL7702的凋亡作用  

Effect of anti-human DR5 monoclonal antibody on the apoptosis of human hepatocyte HL7702 cell lines

作　　者：张军[1] 马远方[1] 刘广超[1] 李淑莲[1] 卢锋[1] 

机构地区：[1]河南大学医学院细胞与分子免疫学重点实验室,河南开封475003

出　　处：《细胞与分子免疫学杂志》2006年第5期641-643,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：河南省杰出人才创新基金项目(0321001800);河南省医学科技创新人才工程资助项目(2002119)

摘　　要：目的:探讨抗人DR5单克隆抗体(mDRA6)对人肝细胞系HL7702致凋亡的作用。方法:流式细胞术检测HL7702细胞表面DR5的表达。在荧光显微镜下观察mDRA6对HL7702细胞形态变化的影响;MTT法检测mDRA6的细胞毒性作用;AnnexinVFITC/PI双染法流式细胞术检测细胞凋亡率。结果:mDRA6导致HL7702细胞呈现典型细胞凋亡的形态特征;MTT法检测显示在40mg/L浓度下可杀伤39%的细胞;经流式细胞术检测显示,3mg/L的mDRA6作用HL7702细胞6h导致25.5%的细胞发生凋亡。结论:mDRA6能够诱导人肝细胞系HL7702凋亡,mDRA6具有诱导细胞凋亡的活性。AIM： To evaluate the effect of a novel antihuman DR5 monoclonal antibody （mAb mDRA-6） on the apoptosis of human hepatocyte HL7702 cell lines. METHODS： DR5 expression on HL7702 cell surface was determined by flow cytometry（FCM）. The effect of mAb mDRA-6 on the morphous of HL7702 cells was observed by fluorenscence microscope, mDRA-6 cytotoxicity on HL7702 cells was detected by using MTT analysis. The rate of apoptosis was detected by FCM with Annexin V-FITC/PI staining. RESULTS： HL7702 cells treated with mDRA-6 exhibited some typical apoptostic features in morphology. MTT analysis showed the death rate of HL7702 cells was 39% in the presence of 40 mg/L mDRA-6 for 6 hours. FCM detection indicated the apoptosis rate of the cells was 25.5% in the presence of 3 mg/L mDRA-6 for 6 hours with Annexin V-FITC/PI staining. CONCLUSION： mDRA-6 can induce the apoptosis of HL7702 cells.

关 键 词：DR5 单克隆抗体 细胞凋亡 

分 类 号：R392.5[医药卫生—免疫学]