动脉粥样硬化兔纤溶酶原激活物抑制物-1的表达及与血脂相关性的研究  

Study on expression of plasminogen activator inhibitor type-1 and its correlation with cholesterolemia in the atherosclerotic rabbits

作　　者：秦玲[1] 祝雁[2] 黄可心[3] 

机构地区：[1]吉林大学第一医院心内科,长春130021 [2]吉林大学前卫医院内科 [3]吉林大学基础医学院组织学与胚胎学教研室

出　　处：《临床心血管病杂志》2006年第9期538-540,共3页Journal of Clinical Cardiology

基　　金：吉林省科技厅课题(No:20050408)

摘　　要：目的:探讨动脉粥样硬化(AS)兔血清和粥样硬化斑块中纤溶酶原激活物抑制物-1(PAI-1)的表达以及与血脂的相关性。方法:雄性大耳白兔随机分成正常饮食组和高脂饮食组,每组8只,饲养16周。2组动物均于饲养0、8、16周采耳缘静脉血,检测血清中胆固醇(TC)、低密度脂蛋白(LDL)以及PAI-1的水平;16周后处死动物,应用免疫组化方法检测PAI-1在主AS斑块中的表达。结果:正常饮食组0、8、16周血清中TC、LDL和PAI-1水平差异无统计学意义(均P>0·05);高脂饮食组8周后血清中TC、LDL和PAI-1水平较0周显著增加(均P<0·01),16周较8周增加更为明显(均P<0·01);免疫组化结果显示高脂饮食组主动脉壁PAI-1的表达明显高于对照组(P<0·01)。结论:AS的发生伴有血清和粥样斑块中PAI-1表达增加,表达量与血清TC、LDL呈正相关。Objective：To study the expression of plasminogenactivator inhibitor type-1（PAI-1）of the blood serum and plaques in atherosclerotic rabbits and its correlation with cholesterol. Method： Male white rabbits were randomly divided into the normal diet group and the high-cholesterol diet group and were fed for 16 weeks. Blood was collected from the marginal vein of the ear at 0, 8 and 16 weeks respectively in two groups. Concentrations of TC, LDL, and PAI-1 were measured. After 16 weeks, rabbits were put to death and the aortas were harvested for immunohistochemistry analysis of PAI-1 expression. Result：There were no obviously differences of TC, LDL and PAI-1 in the normal diet group among 0, 8, and 16 weeks （ P 〉0.01） ,whereas TC,LDL and PAI-1 in the high- cholestrol diet group were significanfly increased after 8 weeks （ P 〈0.01） ,and even higher after 16 weeks （ P 0.01）. Conclusion：The expressions of TC, LDL and PAI-I are increased in blood serum and aortas plaques of atberosclertic rabbits . The expression of PAI-1 has positive correlation with TC and LDL.

关 键 词：动脉粥样硬化 高脂血症 纤溶酶原激活物抑制物-1 

分 类 号：R543.5[医药卫生—心血管疾病]