氧化型低密度脂蛋白的不同组分在诱导巨噬细胞凋亡中的作用  

Role of the Different Components of Oxidized Low Density Lipoprotein in In duction of Apoptosis in Macrophages

作　　者：刘尚喜[1] 周玫[1] 陈瑗[1] 

机构地区：[1]第一军医大学自由基医学研究室

出　　处：《中国动脉硬化杂志》1996年第4期241-245,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金

摘　　要：通过观察凋亡巨噬细胞的特征性ＤＮＡ梯形图谱和细胞核的形态变化，研究了氧化型低密度脂蛋白的不同组份在其诱导的巨噬细胞凋亡中的作用。结果发现：氧化型低密度脂蛋白可引起巨噬细胞凋亡，表现在ＤＮＡ图谱呈典型的梯形，细胞核固缩，正常和乙酰化低密度脂蛋白均未引起巨噬细胞的明显凋亡，用谷胱苷肽过氧化物酶模拟物ｅｂｓｅｌｅｎ清除氧化型低密度脂蛋白上的脂氢过氧化物，可部分抑制氧化型低密度脂蛋白引起的ＤＮＡ断裂。氧化型低密度脂蛋白的脂质组份可引起巨噬细胞ＤＮＡ的明显断裂，而蛋白组份引起的断裂则不明显。低密度脂蛋白脂质的主要成份亚油酸和胆固醇单独氧化后均可引起巨噬细胞ＤＮＡ断裂，二者混合在一起氧化后引起的ＤＮＡ断裂更加明显。Aim To investigate which component in oxidized low density lipoprotein(OLDL)is responsible for the apoptosis in macrophages induced by OLDL , the roles of several components in OLDL in apoptosis in macrophages were studied; Methods LDL was modified by Cu2+,cell nuclei were stained with Hoechst 3 325 8 ; DNA ladder was observed by agarose gel electrophoresis。Results OLDL could induce apoptosis in macrophages characterized by appearance of DNA ladder and nucleus condensation , but native LDL and acetylated LDL (Ac-LDL) could not,Pre-clearance of lipid hydroperoxides(LOOH)in OLDL by ebselen plus GSH could partly inhibit apoptosis induced by OLDL. Lipid moiety in OLDL could induce apparent apoptois, but protein moiety could not.BOth linoleic acid and cholesterol, the main components of LDL lipid,oxidized separately,could induce apoptosis in macrophages.The DNA fragmentation induced by linoleic acid and cholesterol oxidized together was much more apparent than that induced by either of linoleic acid and cholesterol oxidized alone.Conclusions LOOH and oxidized cholesterol in OLDL may be responsible for t he apoptosis in macrophages induced by OLDL.

关 键 词：低密度脂蛋白 巨噬细胞 细胞凋亡 动脉粥样硬化 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R543.502[医药卫生—基础医学]