自体内皮前体细胞移植促缺血心肌血管新生的实验研究  

Experimental studies of implantation of autologous murine endothelial progenitor cells(EPC)to promote neovascularization in ischemic myocardium

作　　者：邢泉生 泮思林 孙龙[2] 

机构地区：[1]青岛儿童心脏中心,山东省青岛266011 [2]青岛市立医院

出　　处：《中华急诊医学杂志》2006年第9期793-797,共5页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金资助(30271294)

摘　　要：目的研究从外周血中获取的内皮前体细胞自体移植后促进心肌缺血区域血管新生的可行性和有效性,欲为冠心病患者的治疗提供新的细胞移植学方法。方法抽取动物的外周动脉血,应用密度梯度离心法获取单个核细胞。应用加入VEGF和bFGF的特定培养基培养后,获得CD31、CD34、Flk-1和vWF免疫荧光染色阳性的内皮前体细胞；结扎SD大鼠冠状动脉左前降支,建立急性心肌梗死模型；然后将得到的自体细胞重新植入缺血心肌局部区域。对照组注入细胞培养液。结果移植组与对照组比较,HE染色显示心肌胶原纤维融合较少,组织排列结构更为有序；移植区域微血管密度明显增高。结论通过一定的体外分离、定向分化、培养、扩增途径,可以从外周血获得较为纯化的内皮前体细胞；内皮前体细胞移植对局部梗死心肌组织结构有一定的保护作用,并可促进血管新生。Objective To explore the procedures of isolation, cultivation of endothelial progenitor cells （EPC） from peripheral bloods and the feasibility of local EPC transplantation to promote neovascularization in ischemic myocardium. Methods Peripheral blood was obtained from the femoral artery of each Sprague-Dawley （SD） rat. Myocardial ischemia was induced by ligation of murine left anterior descending coronary artery. Immmdiate timepoint of ligation procedure, autologous EPC from peripheral blood, positive for CD31, D34, Flk-1 and vWF, isolated from the peripheral blood of each animal were injected to ischemic myocardium. In the control groups, cell culture media was injected. Rats were euthanized and neovascularization in local ischemia areas was evaluated. Results Hematoxylin-eosin staining indicated better myocardial arrangement in the EPC group. Capillary density in ischemic myocardium weeks after transplantation was significantly greater in the group of EPC injection than the control groups. Conclusion Relatively purified EPC can be obtained by certain procedure of isolation and culture from rat peripheral blood. Intramyocardial transplantation of autologous EPCs will promote microangiogenesis and has a protective effect on ischemic myocardial tissue.

关 键 词：内皮前体细胞 细胞移植 血管新生 缺血性心脏病 

分 类 号：R654.2[医药卫生—外科学]