冬虫夏草菌丝对二甲基亚硝胺诱导的大鼠肝纤维化的作用  

Effects of Cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats

作　　者：李风华[1] 刘平[1] 熊卫国[1] 徐光福[2] 

机构地区：[1]上海中医药大学肝病研究所,上海201203 [2]北京中医药大学东直门医院中医科,北京100700

出　　处：《中西医结合学报》2006年第5期514-517,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金杰出青年基金资助项目(No.39825128);上海市重点学科建设资助项目(No.Y0302);上海市高等学校科技基金资助项目(No.01QN78)

摘　　要：目的:探讨冬虫夏草菌丝对二甲基亚硝胺诱导的大鼠肝纤维化的作用。方法:将SD大鼠分为正常对照组、模型组、冬虫夏草菌丝治疗组。采用二甲基亚硝胺诱导的大鼠肝纤维化模型。每组大鼠给予相应的药物。4周后,大鼠肝脏用胶原染色观察胶原纤维沉积的变化,盐酸水解法检测肝组织羟脯氨酸含量,Envi-sion两步法测定肝组织Ⅳ型胶原含量和金属蛋白酶组织抑制因子2(tissueinhibitorofmetalloproteinase-2,TIMP-2)含量;酶图法检测肝组织基质金属蛋白酶2(matrixmetalloproteinases-2,MMP-2)活性,Western印迹法检测肝组织Ⅰ型胶原。结果:模型组大鼠肝组织羟脯氨酸、Ⅳ型胶原和TIMP-2含量,I型胶原蛋白表达,较正常对照组均增加,而MMP-2含量降低;冬虫夏草菌丝治疗组大鼠肝组织羟脯氨酸、Ⅳ型胶原和TIMP-2含量,I型胶原蛋白表达较模型组均有不同程度的下降,而MMP-2含量升高。结论:冬虫夏草菌丝有显著的抗肝纤维化作用,其作用机制与促进胶原降解有关。Objective： To study the effects of Cordyceps sinensis on dimethylnitrosamine-induced liver fibrosis in rats. Methods： SD rats were divided into normal control group, untreated group and Cordyceps sinensis-treated group. The rats in each group were fed with corresponding drug for 4 weeks. The rat＇s liver collagen deposition was observed with collagen staining. Hydroxyproline （Hyp） contents in liver tissue of the rats in 3 groups were determined with HCI hydrolysis. The tissue inhibitor of metalloproteinase-2（TIMP-2） and type Ⅳ collagen contents were observed by Envision, and matrix metalloproteinases-2 （MMP-2） activity was detected by the method of enzyme-picture. Type Ⅰ collagen was detected by Western blotting. Results： The contents of Hyp, TIMP-2, type Ⅳ collagen, and the expression of type Ⅰ collagen in untreated group were significantly higher than those in the normal control group, while those in Cordyceps sinensis-treated group were significantly lower than those in the untreated group. The content of MMP-2 in untreated group was significantly lower than that in the normal control group, while that in Cordyceps sinensis-treated group was significantly higher than that in the untreated group. Conclusion： Cordyceps sinensis can considerably relieve the liver fibrosis, and the mechanism may be related to promoting the degradation of the collagens.

关 键 词：肝纤维化 二甲基亚硝胺 冬虫夏草 菌丝 大鼠 

分 类 号：R285.5[医药卫生—中药学]